| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| Other Sizes |
| Targets |
Cytosine is a component of nucleic acids and pairs with guanine. It is a pyrimidine base that is essential for the storage and transmission of genetic information. It is involved in various biological processes related to DNA and RNA function.
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|---|---|
| ln Vitro |
Cytosine is one of the four main bases found in DNA and RNA. It pairs with guanine through three hydrogen bonds. It is a pyrimidine derivative. It is essential for the structure and function of nucleic acids.
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| ln Vivo |
Cytosine is used in research as a component of nucleic acids and in the study of DNA and RNA structure and function. It is a fundamental building block of life. It is used in various biochemical and molecular biology applications.
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| Enzyme Assay |
Cytosine is used as a substrate or standard in cell-free assays for enzymes involved in nucleic acid metabolism. Its purity and identity can be confirmed using HPLC and NMR analysis.
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| Cell Assay |
Cytosine is used in cell-based assays to study nucleic acid metabolism and gene expression. It is incorporated into DNA and RNA during synthesis. Its effects on cellular function can be studied in various cell culture models.
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| Animal Protocol |
Cytosine is administered in animal models to study its role in nucleic acid metabolism. However, specific animal study protocols are not extensively documented. It is a naturally occurring compound.
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| ADME/Pharmacokinetics |
Cytosine has a molecular weight of 111.10 and a molecular formula of C4H5N3O. It has a CAS number of 71-30-7. The compound is a white solid with a melting point >300°C. It is soluble in acid.
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| Toxicity/Toxicokinetics |
No detailed toxicity data is available for Cytosine. The compound is a naturally occurring nucleobase and is generally considered safe. It is used in research and as a component of nucleic acids.
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| References | |
| Additional Infomation |
Cytosine is an aminopyrimidine, a pyrimidin-2-one with its amino group at position 4. It is a metabolite found in humans, E. coli, Saccharomyces cerevisiae, and mice. It is a pyrimidine nucleobase, a pyrimidinone, and an aminopyrimidine. Cytosine is a metabolite found in or produced by E. coli (K12 strain, MG1655 strain). It has also been reported to be present in tea, soybeans, and other organisms with relevant data. Cytosine is a pyrimidine base found in DNA and RNA, pairing with guanine. Cytosine is a metabolite found in or produced by Saccharomyces cerevisiae. A pyrimidine base, it is a basic building block of nucleic acids. See also: Pyrimidine (subclass).
Cytosine is one of the four main nitrogenous bases found in DNA and RNA. It has a CAS number of 71-30-7. It pairs with guanine and is essential for the structure and function of nucleic acids. It is used in research to study DNA and RNA. It is a naturally occurring compound. |
| Molecular Formula |
C4H5N3O
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|---|---|
| Molecular Weight |
111.1
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| Exact Mass |
111.043
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| CAS # |
71-30-7
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| Related CAS # |
52685-04-8;26297-64-3
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| PubChem CID |
597
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
445.8ºC at 760 mmHg
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| Melting Point |
>300 °C(lit.)
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| Flash Point |
223.4ºC
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| Index of Refraction |
1.689
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| LogP |
-2.29
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
8
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| Complexity |
170
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1N=C([H])C([H])=C(N([H])[H])N1[H]
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| InChi Key |
OPTASPLRGRRNAP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C4H5N3O/c5-3-1-2-6-4(8)7-3/h1-2H,(H3,5,6,7,8)
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| Chemical Name |
6-amino-1H-pyrimidin-2-one
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| Synonyms |
NSC27787; NSC-27787; NSC 27787
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~16.67 mg/mL (~150.05 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (15.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (15.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.0009 mL | 45.0045 mL | 90.0090 mL | |
| 5 mM | 1.8002 mL | 9.0009 mL | 18.0018 mL | |
| 10 mM | 0.9001 mL | 4.5005 mL | 9.0009 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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September 1, 2021 | Not Applicable |
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