| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Acetyl-CoA carboxylase (ACCase) in grasses (herbicide target).
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|---|---|
| ln Vitro |
Cyhalofop inhibits acetyl-CoA carboxylase (ACCase) in susceptible grasses, blocking fatty acid biosynthesis and leading to the death of the plant. It is the active metabolite responsible for the herbicidal activity of Cyhalofop-butyl.
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| ln Vivo |
Cyhalofop is the herbicidally active metabolite that controls grass weeds in rice crops. It is effective against a wide range of grass weed species at various growth stages.
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| Enzyme Assay |
ACCase enzyme activity assays are performed using plant extracts from susceptible grasses. The enzyme is incubated with acetyl-CoA and malonyl-CoA in the presence of the compound. Fatty acid synthesis is measured by incorporation of radiolabeled acetate or by spectrophotometric methods.
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| Cell Assay |
Plant cell cultures or whole plants are treated with Cyhalofop. ACCase activity and lipid synthesis are measured. Plant growth inhibition and visual symptoms of herbicidal activity are assessed.
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| Animal Protocol |
Greenhouse and field trials are conducted to assess the herbicidal efficacy of Cyhalofop-butyl (which is metabolized to Cyhalofop). Grass weed control, crop safety, and yield are evaluated in rice crops.
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| ADME/Pharmacokinetics |
As a herbicide, Cyhalofop is applied to crops and is metabolized from the butyl ester prodrug to the active acid form. It has low mammalian toxicity and is rapidly excreted. The compound has low persistence in the environment.
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| Toxicity/Toxicokinetics |
Low toxicity to mammals and non-target organisms. The compound is classified as having low acute oral toxicity. No significant genotoxicity or carcinogenicity concerns have been identified.
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| References |
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| Additional Infomation |
Cyhalofop is an agricultural herbicide used worldwide for grass weed control in rice. It is not a pharmaceutical drug and has no human therapeutic applications. The compound is registered for agricultural use in many countries including India.
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| Molecular Formula |
C16H12FNO4
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|---|---|
| Molecular Weight |
301.26918
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| Exact Mass |
301.075
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| CAS # |
122008-78-0
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| PubChem CID |
15118048
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| Appearance |
White to off-white solid powder
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| LogP |
4.6
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
22
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| Complexity |
427
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C[C@H](C(=O)O)OC1=CC=C(C=C1)OC2=C(C=C(C=C2)C#N)F
|
| InChi Key |
ROBSGBGTWRRYSK-SNVBAGLBSA-N
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| InChi Code |
InChI=1S/C16H12FNO4/c1-10(16(19)20)21-12-3-5-13(6-4-12)22-15-7-2-11(9-18)8-14(15)17/h2-8,10H,1H3,(H,19,20)/t10-/m1/s1
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| Chemical Name |
(2R)-2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 31 mg/mL (~102.90 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (6.90 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3193 mL | 16.5964 mL | 33.1928 mL | |
| 5 mM | 0.6639 mL | 3.3193 mL | 6.6386 mL | |
| 10 mM | 0.3319 mL | 1.6596 mL | 3.3193 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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