Size | Price | Stock | Qty |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
Cyclizine (100 μM) dramatically reduced the levels of iNOS protein and LPS-stimulated nitrite buildup in RAW 264.7 cell supernatants [1]. With an IC50 of 5.42 µM, cyclidine prevents anti-IgE-induced histamine release from human lung fragments[2].
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ln Vivo |
Mice's locomotor activity is enhanced by cycliclizine (1, 10 mg/kg) in a dose-dependent way [3].
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Animal Protocol |
Animal/Disease Models: Naive male CDI mice (Charles River), body weight 25-30 g[3]
Doses: 1, 10 mg/kg Route of Administration: subcutaneous injection; measure locomotor activity (crossover) every 15 minutes for 2 hrs (hrs (hours)) . Experimental Results: Enhanced locomotor activity in a dose-dependent manner. |
References |
[1]. Králová J, et, al. The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity. Int Immunopharmacol. 2009 Jul;9(7-8):990-5.
[2]. Church MK, et, al. Inhibition of histamine release from human lung in vitro by antihistamines and related drugs. Br J Pharmacol. 1980 Aug;69(4):663-7. [3]. Leza JC, et, al. Effects of antihistaminics on locomotor activity in mice. Comparison with opiate and amphetamine-induced hyperactivity. Gen Pharmacol. 1991;22(2):293-6. |
Molecular Formula |
C18H22N2
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Molecular Weight |
266.38
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Exact Mass |
266.1783
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CAS # |
82-92-8
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Related CAS # |
Cyclizine dihydrochloride;5897-18-7;Cyclizine lactate;5897-19-8;Cyclizine hydrochloride;303-25-3
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SMILES |
CN1CCN(CC1)C(C2=CC=CC=C2)C3=CC=CC=C3
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Chemical Name |
1-benzhydryl-4-methylpiperazine
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Synonyms |
NautazineCYCLIZINE Valoid Neo-devomitMarezine Ciclizina
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~375.40 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.7540 mL | 18.7702 mL | 37.5404 mL | |
5 mM | 0.7508 mL | 3.7540 mL | 7.5081 mL | |
10 mM | 0.3754 mL | 1.8770 mL | 3.7540 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.