| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
CY3-YNE does not have a specific biological target; it is a chemical tool for bioconjugation. The alkyne group serves as a bioorthogonal handle for selective conjugation to azide-functionalized molecules via CuAAC click chemistry. Its primary "target" is the chemical functionality of azide-containing compounds. The cyanine fluorophore provides fluorescence for detection and imaging applications.
|
|---|---|
| ln Vitro |
CY3-YNE's in vitro activity is assessed by its efficiency as a click chemistry reagent. It is used to label biomolecules such as proteins, peptides, and oligonucleotides. The labeling efficiency is typically evaluated by measuring fluorescence intensity after conjugation. The compound's reactivity in CuAAC reactions is monitored by HPLC, LC-MS, or fluorescence spectroscopy. CY3-YNE exhibits good photostability and bright fluorescence, making it suitable for various bioanalytical assays.
|
| ln Vivo |
In vivo applications of CY3-YNE are primarily related to its use in bioorthogonal labeling for imaging. The compound can be conjugated to targeting ligands or nanoparticles for in vivo fluorescence imaging. Its sulfonated structure may limit cell permeability but improves water solubility. Detailed in vivo activity data are limited as the compound is primarily a chemical reagent.
|
| Enzyme Assay |
CY3-YNE is not a drug targeting biological macromolecules, so standard enzyme/receptor binding assays are not applicable. Instead, its chemical reactivity is assessed using CuAAC click chemistry reactions. Typical protocols involve mixing the compound with an azide-functionalized molecule in the presence of a copper catalyst (e.g., CuSO4) and a reducing agent (e.g., sodium ascorbate) at room temperature or 37degC. Reaction progress is monitored by TLC, HPLC, or mass spectrometry. Conjugation efficiency is calculated by comparing the relative amounts of starting materials and products.
|
| Cell Assay |
Cellular assays for CY3-YNE involve evaluating its utility for cell surface or intracellular labeling. Cells are incubated with azide-modified biomolecules (e.g., sugars, proteins) to incorporate azide groups metabolically, followed by reaction with CY3-YNE via click chemistry. Labeling is detected by fluorescence microscopy or flow cytometry. Cytotoxicity of the compound or its conjugates is assessed using standard cell viability assays such as MTT. The compound's labeling specificity is evaluated by comparing fluorescence signals in treated versus control cells.
|
| Animal Protocol |
In vivo animal studies for CY3-YNE are limited, as the compound is primarily a chemical reagent. When used in vivo, the compound is typically conjugated to targeting moieties and administered to animal models for fluorescence imaging studies. Tissue distribution and labeling efficiency are assessed by imaging or tissue analysis. The compound may be used in pre-targeting strategies where azide-modified antibodies or nanoparticles are administered first, followed by CY3-YNE for bioorthogonal labeling. Detailed in vivo protocols are not extensively reported.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of CY3-YNE are characteristic of fluorescent cyanine dyes. The compound has a molecular weight of 667.84 and a molecular formula of C34H41N3O₇S2. The sulfonate groups enhance aqueous solubility. The compound is typically stored at -20degC, protected from light. Its pharmacokinetic properties are highly dependent on the specific conjugate to which it is attached. Detailed PK parameters such as half-life and bioavailability are not extensively reported for this reagent.
|
| Toxicity/Toxicokinetics |
CY3-YNE is a research reagent and not intended for human therapeutic use. The compound contains an alkyne group, which is generally stable under physiological conditions. In cell-based assays, the compound is typically well-tolerated at concentrations used for labeling. The cyanine fluorophore may have some phototoxicity upon prolonged light exposure. Standard safety precautions should be followed when handling this compound.
|
| Additional Infomation |
CY3-YNE is a click chemistry reagent and fluorescent dye widely used in chemical biology and bioconjugation. Its applications include protein labeling, peptide labeling, oligonucleotide/DNA labeling, fluorescence imaging, and molecular tracking. The compound is compatible with copper-catalyzed azide-alkyne cycloaddition (CuAAC). It is not an FDA-approved drug and has no clinical indications. The compound is available in high purity and is typically stored at -20degC, protected from light.
|
| Molecular Formula |
C34H41N3O7S2
|
|---|---|
| Molecular Weight |
667.835246801376
|
| Exact Mass |
667.238
|
| CAS # |
1010386-62-5
|
| PubChem CID |
124220414
|
| Appearance |
Brown to reddish brown solid powder
|
| LogP |
3.8
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
11
|
| Heavy Atom Count |
46
|
| Complexity |
1490
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
S(C1C=CC2=C(C=1)C(C)(C)C(C=CC=C1C(C)(C)C3C=C(C=CC=3N1CC)S(=O)(=O)[O-])=[N+]2CCCCCC(NCC#C)=O)(=O)(=O)O
|
| InChi Key |
DVBDEKRWCVSXGU-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C34H41N3O7S2/c1-7-20-35-32(38)15-10-9-11-21-37-29-19-17-25(46(42,43)44)23-27(29)34(5,6)31(37)14-12-13-30-33(3,4)26-22-24(45(39,40)41)16-18-28(26)36(30)8-2/h1,12-14,16-19,22-23H,8-11,15,20-21H2,2-6H3,(H2-,35,38,39,40,41,42,43,44)
|
| Chemical Name |
2-[3-[3,3-dimethyl-1-[6-oxo-6-(prop-2-ynylamino)hexyl]-5-sulfoindol-1-ium-2-yl]prop-2-enylidene]-1-ethyl-3,3-dimethylindole-5-sulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~37.38 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.83 mg/mL (1.24 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4974 mL | 7.4868 mL | 14.9736 mL | |
| 5 mM | 0.2995 mL | 1.4974 mL | 2.9947 mL | |
| 10 mM | 0.1497 mL | 0.7487 mL | 1.4974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
