| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
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| Targets |
CY3 is a fluorescent labeling reagent that binds to target molecules (e.g., proteins, antibodies, nucleic acids) through reactive groups such as NHS esters, maleimides, or azides. The dye has an excitation maximum around 550–555 nm and an emission maximum around 570–575 nm, making it compatible with standard Cy3/TRITC filter sets. It is used for labeling biomolecules for fluorescence microscopy, flow cytometry, and FRET-based assays.
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| ln Vitro |
CY3 exhibits bright fluorescence with high quantum yield and photostability. In vitro, the dye is used to label antibodies, peptides, proteins, and nucleic acids for detection and quantification. The fluorescence intensity is stable over a wide pH range and resistant to photobleaching, making it suitable for long-term imaging applications.
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| ln Vivo |
In vivo, CY3-labeled probes are used for fluorescence imaging in small animal models. The dye's near-infrared fluorescence properties (depending on the specific Cy dye variant) allow for deep tissue imaging with minimal autofluorescence. CY3-labeled antibodies or nanoparticles can be used for tumor imaging, biodistribution studies, and tracking of cells or biomolecules in vivo.
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| Enzyme Assay |
For labeling reactions, CY3-NHS ester is dissolved in anhydrous DMSO or DMF and reacted with amine-containing biomolecules (proteins, peptides, amine-modified oligonucleotides) in bicarbonate buffer (pH 8.3–9.0) at room temperature for 1–4 hours. Unreacted dye is removed by dialysis, gel filtration, or HPLC. Labeling efficiency is assessed by absorbance at 550 nm and protein concentration (A280). The degree of labeling (DOL) is calculated from the absorbance ratio.
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| Cell Assay |
For cellular assays, cells are incubated with CY3-labeled probes (e.g., antibodies, ligands, nanoparticles) at appropriate concentrations for 30 minutes to several hours at 4°C or 37°C. After washing, fluorescence is measured by flow cytometry, fluorescence microscopy, or a fluorescence microplate reader. For live-cell imaging, cells are maintained in appropriate media and imaged using confocal or widefield fluorescence microscopy.
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| Animal Protocol |
In vivo imaging is performed using small animal fluorescence imaging systems (e.g., IVIS, Pearl Imager). CY3-labeled probes are administered via intravenous, intraperitoneal, or local injection. Animals are anesthetized and imaged at various time points post-injection using appropriate excitation (550–555 nm) and emission (570–575 nm) filters. Fluorescence signal is quantified and analyzed using imaging software.
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| ADME/Pharmacokinetics |
CY3 is a hydrophilic, sulfonated dye with good water solubility, reducing non-specific binding to biological membranes. The dye is stable in aqueous solutions and resistant to photobleaching. Pharmacokinetics of CY3-labeled probes depend on the conjugated biomolecule rather than the dye itself. Unconjugated CY3 is rapidly cleared from circulation via renal excretion.
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| Toxicity/Toxicokinetics |
CY3 is generally considered non-toxic for labeling applications. The dye itself has low cytotoxicity, though the toxicity of CY3-labeled probes depends on the conjugated biomolecule. Standard safety precautions should be followed when handling the dye (avoid inhalation, skin contact, and eye contact). The compound is for research use only and not for human diagnostic or therapeutic use.
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| Additional Infomation |
CY3 is a sulfonated indocarbocyanine fluorophore widely used for fluorescence labeling and imaging. It has an excitation maximum at 550–555 nm and emission at 570–575 nm, compatible with standard Cy3/TRITC filter sets. The dye is used to label proteins, antibodies, and nucleic acids for fluorescence microscopy, flow cytometry, and in vivo imaging.
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| Molecular Formula |
C31H38N2O8S2
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|---|---|
| Molecular Weight |
630.772026538849
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| CAS # |
146368-13-0
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| PubChem CID |
91811029
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| Appearance |
Purple to purplish red solid powder
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| LogP |
6.959
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
43
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| Complexity |
1360
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~12.5 mg/mL (~19.82 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (39.63 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5854 mL | 7.9268 mL | 15.8536 mL | |
| 5 mM | 0.3171 mL | 1.5854 mL | 3.1707 mL | |
| 10 mM | 0.1585 mL | 0.7927 mL | 1.5854 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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