| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
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| ln Vitro |
Curzerene (0-100 µM; 24-72 hours) showed dose- and time-dependently increasing cytostatic effects against SPC A1 cells with IC50s of 403.8 µM, 154.8 µM, and 47.01 µM at 24, 48, and 72 hours, respectively [1]. In comparison to controls, Curzerene (0-100 µM; 48 hours) causes greater proportions of necrotic and apoptotic cells in SPC-A1 cells [1]. The percentage of cells arrested in the G2/M phase rose from 9.26% of cells in the control group to 17.57% of cells treated with the highest dose, according to research using curzerene (0-100 µM; 48 hours) [1]. In SPC A1 cells, curzerene (6.25-100 µM; 48 hours) decreases GSTA1 mRNA expression [1]. In SPC A1 cells, curzerene (6.25-100 µM; 48 hours) can lower GSTA1 protein expression [1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: SPC-A1 Cell Tested Concentrations: 0 µM, 6.25 µM, 12.5 µM, 25 µM, 50 µM, 100 µM Incubation Duration: 24 hrs (hours), 48 hrs (hours), 72 hrs (hours) Experimental Results: Inhibition of non-cell growth In vitro experiments on lung cancer SPC A1 cells. Apoptosis analysis[1] Cell Types: SPC-A1 Cell Tested Concentrations: 0 µM, 6.25 µM, 12.5 µM, 25 µM, 50 µM, 100 µM Incubation Duration: 48 hrs (hours) Experimental Results: Induction of apoptosis in a dose-dependent manner. Apoptosis analysis[1] Cell Types: SPC-A1 Cell Tested Concentrations: 0 µM, 6.25 µM, 12.5 µM, 25 µM, 50 µM, 100 µM Incubation Duration: 48 hrs (hours) Experimental Results: Induction of G2/M cell cycle in SPC A1 cells Stagnation. RT-PCR[1] Cell Types: SPC-A1 Cell Tested Concentrations: 6.25 µM, 25 µM, 100 µM Incubation Duration: 48 hrs (hours) Experimental Results: diminished GSTA1 mRNA expression. |
| References | |
| Additional Infomation |
Benzofuran, 6-ethenyl-4,5,6,7-tetrahydro-3,6-dimethyl-5-isopropenyl-, trans- has been reported in Curcuma xanthorrhiza, Lindera pulcherrima var. hemsleyana, and other organisms with data available.
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| Molecular Formula |
C15H20O
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|---|---|
| Molecular Weight |
216.3187
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| Exact Mass |
216.151
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| CAS # |
17910-09-7
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| PubChem CID |
12305301
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
282.8±40.0 °C at 760 mmHg
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| Flash Point |
117.5±14.2 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.539
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| LogP |
5.78
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
16
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| Complexity |
307
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| Defined Atom Stereocenter Count |
2
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| SMILES |
O1C([H])=C(C([H])([H])[H])C2=C1C([H])([H])C(C([H])=C([H])[H])(C([H])([H])[H])C([H])(C(=C([H])[H])C([H])([H])[H])C2([H])[H]
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| InChi Key |
HICAMHOOTMOHPA-HIFRSBDPSA-N
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| InChi Code |
InChI=1S/C15H20O/c1-6-15(5)8-14-12(11(4)9-16-14)7-13(15)10(2)3/h6,9,13H,1-2,7-8H2,3-5H3/t13-,15+/m1/s1
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| Chemical Name |
(5R,6R)-6-ethenyl-3,6-dimethyl-5-prop-1-en-2-yl-5,7-dihydro-4H-1-benzofuran
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. (3). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1155.70 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 6.25 mg/mL (28.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 6.25 mg/mL (28.89 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 62.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 6.25 mg/mL (28.89 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6228 mL | 23.1139 mL | 46.2278 mL | |
| 5 mM | 0.9246 mL | 4.6228 mL | 9.2456 mL | |
| 10 mM | 0.4623 mL | 2.3114 mL | 4.6228 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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