| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
CPPHA alone has no agonist action [1]. CPPHA promotes a concentration-dependent increase of the responsiveness of human mGluR5 CHO cells to agonists [1]. CPPHA can boost the threshold response to glutamate in fluorescent Ca2+ assays by 7 to 8 times, with EC50 values in the range of 400 to 800 nM [1]. CPPHA (20 μM; 15 min) improves the reaction to subthreshold doses of DHPG on ERK and cyclic AMP response element binding protein (CREB) activity, as well as NMDA receptor subunit NR1 phosphorylation in cortical and hippocampal slices [3] .
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|---|---|
| Cell Assay |
Western blot analysis [3]
Cell Types: Rat hippocampal cells Tested Concentrations: 20 μM Incubation Duration: 15 minutes Experimental Results: Enhanced DHPG (20 μM)-induced increase in ERK and CREB phosphorylation, approaching levels observed with 100 μM DHPG alone . |
| References |
|
| Molecular Formula |
C22H15N2O4CL
|
|---|---|
| Molecular Weight |
406.8185
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| Exact Mass |
406.072
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| CAS # |
693288-97-0
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| PubChem CID |
9931205
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| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.506g/cm3
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| Boiling Point |
539.7ºC at 760 mmHg
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| Flash Point |
280.201ºC
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| Index of Refraction |
1.733
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| LogP |
4.105
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
29
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| Complexity |
633
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
UFOUABRZSDGGAZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H15ClN2O4/c23-14-9-10-18(24-20(27)17-7-3-4-8-19(17)26)13(11-14)12-25-21(28)15-5-1-2-6-16(15)22(25)29/h1-11,26H,12H2,(H,24,27)
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| Chemical Name |
N-[4-chloro-2-[(1,3-dioxoisoindol-2-yl)methyl]phenyl]-2-hydroxybenzamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~245.81 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4581 mL | 12.2904 mL | 24.5809 mL | |
| 5 mM | 0.4916 mL | 2.4581 mL | 4.9162 mL | |
| 10 mM | 0.2458 mL | 1.2290 mL | 2.4581 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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