| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
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| ln Vitro |
Coniferyl aldehyde (0.1–5 μM; generated for 1 hour, then treated for 24 hours) strongly reduces LPS-induced NO generation and cell death in a quantitative coupling manner, while also acting as a cytoprotective against LPS-induced cell death [1]. (0.5–5 μM; 4–24 hours) dramatically boosts the expression of HO-1 and Nrf-2 nuclear translocation. Furthermore, coniferyl aldehyde raises PKCα/PKCβ II phosphorylation [1]. [1]
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|---|---|
| ln Vivo |
In knee articular cartilage, coniferaldehyde (0.05 mmol kg/day; i.p.; 6 weeks) stimulates Nrf2 signaling immunity and sustained primary chondrocytes. In OA arthritis, coniferaldehyde can lessen cartilage degradation [2].
|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: Raw264.7 cells induced with LPS Tested Concentrations: 0.1μM, 0.5μM, 1μM, 2μM, 5μM Incubation Duration: 1 hour pretreatment, then 24 hrs (hours) treatment Experimental Results: Inhibition of LPS-induced NO production and cell death. Western Blot Analysis[1] Cell Types: Raw264.7 Cell Tested Concentrations: 0.5 μM, 1 μM, 2 μM, 5 μM Incubation Duration: 4 hrs (hours), 8 hrs (hours), 12 hrs (hours), 24 hrs (hours) Experimental Results: HO-1 protein levels increased dose and time dependent manner. |
| Animal Protocol |
Animal/Disease Models: B6 male mice (26 g; 8-10 weeks old), surgically induced osteoarthritis (OA) [2]
Doses: 0.05 mmol kg/day (approximately 8.9 mg/kg) Route of Administration: intraperitoneal (ip) injection ; Sustained for 6 weeks Experimental Results: Dramatically diminished medial meniscus cartilage damage in unstable mice. |
| References |
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| Additional Infomation |
Coniferyl aldehyde is a member of the class of cinnamaldehydes that is cinnamaldehyde substituted by a hydroxy group at position 4 and a methoxy group at position 3. It has a role as an antifungal agent and a plant metabolite. It is a member of cinnamaldehydes, a phenylpropanoid and a member of guaiacols. It is functionally related to an (E)-cinnamaldehyde.
4-Hydroxy-3-methoxycinnamaldehyde has been reported in Camellia sinensis, Magnolia officinalis, and other organisms with data available. |
| Molecular Formula |
C10H10O3
|
|---|---|
| Molecular Weight |
178.1846
|
| Exact Mass |
178.062
|
| CAS # |
458-36-6
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| PubChem CID |
5280536
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| Appearance |
Light yellow to brown solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
338.8±27.0 °C at 760 mmHg
|
| Melting Point |
80-82ºC(lit.)
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| Flash Point |
136.8±17.2 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.593
|
| LogP |
1.35
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
|
| Heavy Atom Count |
13
|
| Complexity |
189
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
COC1=C(C=CC(=C1)/C=C/C=O)O
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| InChi Key |
DKZBBWMURDFHNE-NSCUHMNNSA-N
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| InChi Code |
InChI=1S/C10H10O3/c1-13-10-7-8(3-2-6-11)4-5-9(10)12/h2-7,12H,1H3/b3-2+
|
| Chemical Name |
(E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enal
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~561.23 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (5.61 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (5.61 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1 mg/mL (5.61 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6123 mL | 28.0615 mL | 56.1230 mL | |
| 5 mM | 1.1225 mL | 5.6123 mL | 11.2246 mL | |
| 10 mM | 0.5612 mL | 2.8062 mL | 5.6123 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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