| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| Other Sizes |
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| ln Vitro |
LPS/IFN-γ-induced NO generation was reduced by Columbin or l-NAME treatment without compromising RAW264.7 survival. Columbin pretreatment of activated cells did not prevent LPS-stimulated cells' NF-κB translocation to the nucleus. Columbin exhibited 63.7±6.4% and 18.8±1.5% COX-1 and COX-2 inhibitory actions at 100μM, respectively. Columbin's interaction with Tyr385 and Arg120 implies that it is more active in COX-2. Tyr385 is known to be involved in the abstraction of hydrogen from arachidonic acid's C-13, while Arg120 has a high affinity for binding to arachidonic acid, which is highly crucial [1].
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|---|---|
| ln Vivo |
Mice's development of paw edema is inhibited by colonphin. Columbin reduced inflammation for 0 to 5 hours at dosages of 300 mg/kg and 700 mg/kg, with effects similar to those of aspirin, a common anti-inflammatory medication. Columbin may have an inhibitory effect on edema caused by carrageenan in mice's paws because it inhibits the release of inflammatory mediators, such as prostaglandins [1]. Although columbin has a low bioavailability in rats (2.8% orally, 14% i.p.), its quick transit in Caco-2 cell monolayers raises the possibility that the liver's significant first-pass metabolism is to blame for this low bioavailability[2].
|
| References |
|
| Additional Infomation |
Unii-kki91P85GE has been reported in Rehmannia glutinosa, Chasmanthera dependens, and other organisms with data available.
|
| Molecular Formula |
C20H22O6
|
|---|---|
| Molecular Weight |
358.3851
|
| Exact Mass |
358.141
|
| CAS # |
546-97-4
|
| Related CAS # |
Isocolumbin
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| PubChem CID |
188289
|
| Appearance |
White to off-white solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
565.9±50.0 °C at 760 mmHg
|
| Melting Point |
190-191ºC
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| Flash Point |
296.0±30.1 °C
|
| Vapour Pressure |
0.0±1.6 mmHg at 25°C
|
| Index of Refraction |
1.593
|
| LogP |
0.58
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
26
|
| Complexity |
702
|
| Defined Atom Stereocenter Count |
7
|
| SMILES |
C[C@@]12CC[C@H]3C(=O)O[C@@H](C[C@]3([C@@H]1[C@@H]4C=C[C@]2(C(=O)O4)O)C)C5=COC=C5
|
| InChi Key |
AALLCALQGXXWNA-DURQJQQASA-N
|
| InChi Code |
InChI=1S/C20H22O6/c1-18-9-14(11-5-8-24-10-11)25-16(21)12(18)3-6-19(2)15(18)13-4-7-20(19,23)17(22)26-13/h4-5,7-8,10,12-15,23H,3,6,9H2,1-2H3/t12-,13-,14-,15-,18+,19+,20+/m0/s1
|
| Chemical Name |
(1S,2S,3S,5S,8R,11R,12S)-5-(furan-3-yl)-12-hydroxy-3,11-dimethyl-6,14-dioxatetracyclo[10.2.2.02,11.03,8]hexadec-15-ene-7,13-dione
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~279.03 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7903 mL | 13.9513 mL | 27.9026 mL | |
| 5 mM | 0.5581 mL | 2.7903 mL | 5.5805 mL | |
| 10 mM | 0.2790 mL | 1.3951 mL | 2.7903 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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