| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg | |||
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| ln Vitro |
- Against celery pathogens (including Botrytis cinerea and Fusarium oxysporum), Columbianetin showed dose-dependent inhibitory activity on mycelial growth and spore germination. At a concentration of 20 μg/mL, it inhibited the mycelial growth of Botrytis cinerea by 68% and the spore germination rate by 72%; at the same concentration, it inhibited the mycelial growth of Fusarium oxysporum by 59% [1]
- In human mast cells (HMC-1 cell line) activated by phorbol 12-myristate 13-acetate (PMA, 10 nM) and calcium ionophore A23187 (1 μM), Columbianetin (1, 10, 30 μM) dose-dependently inhibited the release of pro-inflammatory mediators. At 30 μM, it reduced tumor necrosis factor-α (TNF-α) release by 62%, interleukin-6 (IL-6) release by 58%, and interleukin-8 (IL-8) release by 51% compared with the activated control group. It also inhibited histamine release by 45% at 30 μM [2] - Columbianetin (10, 30 μM) suppressed the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways in activated HMC-1 cells: it reduced the phosphorylation of IκBα (inhibitor of NF-κB) by 70% at 30 μM, and decreased the phosphorylation levels of extracellular signal-regulated kinase (ERK) and p38 MAPK by 65% and 60%, respectively, at the same concentration [2] |
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| Cell Assay |
- For human mast cell (HMC-1) experiment: HMC-1 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin, maintained at 37°C in a 5% CO₂ incubator. Cells were seeded in 24-well plates at a density of 5×10⁵ cells/well and preincubated with Columbianetin (1, 10, 30 μM) for 1 hour. Then, the cells were stimulated with a combination of PMA (10 nM) and A23187 (1 μM) for 24 hours. After stimulation, the cell supernatant was collected by centrifugation. The levels of TNF-α, IL-6, and IL-8 in the supernatant were detected using enzyme-linked immunosorbent assay (ELISA) kits, and the histamine level was measured by a fluorometric assay. For Western blot analysis, cells were lysed with RIPA buffer containing protease and phosphatase inhibitors, and the lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), then transferred to polyvinylidene fluoride (PVDF) membranes. The membranes were probed with primary antibodies against phosphorylated IκBα (p-IκBα), phosphorylated ERK (p-ERK), phosphorylated p38 (p-p38), and β-actin (loading control), followed by incubation with secondary antibodies. The bands were visualized using an enhanced chemiluminescence (ECL) detection system [2]
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| Toxicity/Toxicokinetics |
In HMC-1 cell viability assay (MTT assay), high concentrations of 30 μM of columbin did not show significant cytotoxicity after 24 hours of treatment; compared with the untreated control group, cell viability remained above 90% [2].
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| References | |
| Additional Infomation |
(S)-Columbin is the (S)-(+)-enantiomer of columbin and also the enantiomer of (R)-Columbin.
Columbin has been reported to be present in Zanthoxylum bungeanum, Curvularia pubescens, and other organisms with relevant data. - Columbin is a phytoalexin that is produced naturally during celery storage. Its accumulation in celery tissues is closely associated with celery’s resistance to postharvest pathogen infections, such as those caused by Botrytis cinerea and Fusarium oxysporum [1]. - Columbin’s anti-inflammatory effect on activated human mast cells is mainly achieved by inhibiting the NF-κB and MAPK signaling pathways, thereby reducing the release of pro-inflammatory cytokines and histamine. This suggests its potential application value in treating inflammatory diseases associated with mast cell activation, such as allergic diseases [2] -Columbin is a coumarin compound whose molecular structure contains a coumarin core and a hydroxyl group at a specific position, which is considered to be related to its biological activities (antibacterial and anti-inflammatory) [1][2] |
| Molecular Formula |
C14H14O4
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|---|---|
| Molecular Weight |
246.2586
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| Exact Mass |
246.089
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| CAS # |
3804-70-4
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| Related CAS # |
(+)-Columbianetin acetate;23180-65-6
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| PubChem CID |
92201
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
443.0±45.0 °C at 760 mmHg
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| Flash Point |
171.9±22.2 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.611
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| LogP |
1.69
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
18
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| Complexity |
387
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(C)([C@@H]1CC2=C(O1)C=CC3=C2OC(=O)C=C3)O
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| InChi Key |
YRAQEMCYCSSHJG-NSHDSACASA-N
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| InChi Code |
InChI=1S/C14H14O4/c1-14(2,16)11-7-9-10(17-11)5-3-8-4-6-12(15)18-13(8)9/h3-6,11,16H,7H2,1-2H3/t11-/m0/s1
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| Chemical Name |
(8S)-8-(2-hydroxypropan-2-yl)-8,9-dihydrofuro[2,3-h]chromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~406.07 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0607 mL | 20.3037 mL | 40.6075 mL | |
| 5 mM | 0.8121 mL | 4.0607 mL | 8.1215 mL | |
| 10 mM | 0.4061 mL | 2.0304 mL | 4.0607 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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