| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Clofentezine targets mite growth and development. It acts as a growth inhibitor, primarily affecting the egg stage (ovicide). It also has some activity on early motile stages. Its mechanism of action involves the inhibition of chitin synthesis, as it is lethal to wild-type, but not chitin synthase 1 (chs1) mutant, mites.
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|---|---|
| ln Vitro |
In vitro, Clofentezine is a growth inhibitor that is highly lethal to mites. Its activity can be assessed in bioassays using mite eggs and larvae. The compound's effects on mite development and survival are measured.
|
| ln Vivo |
In vivo, Clofentezine is used in agriculture for the residual control of mites. It is effective against various plant-feeding mite species, including those of the genera Panonychus and Tetranychus. It provides long-lasting control of mite populations.
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| Enzyme Assay |
Clofentezine is evaluated in cell-free assays to measure its effects on chitin synthesis. However, specific enzymatic assays are not well-documented. Its activity is primarily assessed in whole-organism bioassays using mites.
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| Cell Assay |
Clofentezine is assessed in cell-based assays using mite cell lines or tissues. However, specific cell-based assay protocols are not extensively documented. Its activity is primarily assessed in whole-organism bioassays.
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| Animal Protocol |
Clofentezine is applied to crops in agricultural settings to control mite populations. It is used on a wide range of crops, including apples, pears, stone fruit, nuts, ornamentals, and almonds. Efficacy is assessed by measuring mite population reduction and crop damage.
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| ADME/Pharmacokinetics |
Clofentezine has a molecular weight of 303.15 and a molecular formula of C14H8Cl2N4. It has a CAS number of 74115-24-5. The compound is a solid. It should be stored under recommended conditions.
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| Toxicity/Toxicokinetics |
Toxicity Data
Oral (Rat) > 5200 mg/kg Dermal Contact (Rat) > 2100 mg/kg Inhalation (Rat) > 0.89 (mg/L) No detailed toxicity data is available for Clofentezine. The compound is a pesticide and is not intended for human therapeutic use. It is used in agricultural applications. |
| Additional Infomation |
Clofentezine is a tetrazine compound with the structure 1,2,4,5-tetrazine, in which two hydrogen atoms are substituted with o-chlorophenyl groups. It can be used as a mite growth regulator and a tetrazine acaricide. It is an organochlorine acaricide belonging to the monochlorobenzene and tetrazine classes. It is derived from the hydride of 1,2,4,5-tetrazine. Clofentezine is an acaricide used for residual mite control. It primarily functions as an ovicidal agent, but also has some activity against mites in their early active stages. It is widely used on various crops, such as apples, pears, stone fruits, nuts, ornamental plants, and almonds. In studies on rats and dogs, the main toxicological effects observed were hepatotoxicity and thyroid changes, including follicular hyperplasia. The toxic risk of Clofentezine to humans is not high.
Clofentezine is also known as Apollo and Ovation. It is a tetrazine-class acaricide. It is not approved for human therapeutic use. |
| Molecular Formula |
C14H8CL2N4
|
|---|---|
| Molecular Weight |
303.15
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| Exact Mass |
302.013
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| CAS # |
74115-24-5
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| PubChem CID |
73670
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.398g/cm3
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| Boiling Point |
504.4ºC at 760 mmHg
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| Melting Point |
182-185 °C(lit.)
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| Flash Point |
290.4ºC
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| Index of Refraction |
1.634
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| LogP |
3.907
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
20
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| Complexity |
280
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ClC1=CC=CC=C1C2=NN=C(C3=CC=CC=C3Cl)N=N2
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| InChi Key |
UXADOQPNKNTIHB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H8Cl2N4/c15-11-7-3-1-5-9(11)13-17-19-14(20-18-13)10-6-2-4-8-12(10)16/h1-8H
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| Chemical Name |
3,6-bis(2-chlorophenyl)-1,2,4,5-tetrazine
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| Synonyms |
Panatac; Apollo; Clofnetezine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~41.23 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.12 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2987 mL | 16.4935 mL | 32.9870 mL | |
| 5 mM | 0.6597 mL | 3.2987 mL | 6.5974 mL | |
| 10 mM | 0.3299 mL | 1.6493 mL | 3.2987 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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