| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| Other Sizes |
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| ln Vitro |
Osteoclasts are induced to function swiftly by clodronate disodium salt, which blocks the absorption, internalization, and translocation of ADP into the mitochondria. Consequently, the inhibition of ATP generation causes the release of cytochrome C into the cytoplasm, which causes cellular fluorescence [2].
|
|---|---|
| ln Vivo |
At doses of 12.5 and 25 mg/kg, clodronate disodium salt (6.25–25 mg/kg; lateral; once daily for 28 days) somewhat reduced hindlimb edema [3].
|
| Animal Protocol |
Animal/Disease Models: Male Lewis rat, 7 years of age [3]
Doses: 6.25, 12.5, and 25 mg/kg Route of Administration: Oral; one time/day for 28 days Experimental Results: 25 mg/kg on days 21 and 28 Hind paw swelling was Dramatically less in the adjuvant arthritis (AA) control group (87% and 88% of the AA control group, respectively) and on day 28 in the 12.5 mg/kg dose group. |
| References |
|
| Additional Infomation |
Clodronic acid disodium salt is the disodium salt of clodronic acid. It inhibits bone resorption and soft tissue calcification, and is used (generally as the tetrahydrate) as an adjunct in the treatment of severe hypercalcaemia associated with malignancy, and in the management of osteolytic lesions and bone pain associated with skeletal metastases. It has a role as a bone density conservation agent. It is an organic sodium salt and a one-carbon compound. It contains a clondronate(2-).
Clodronate Disodium is the disodium salt of a nitrogen-free bisphosphonate analog of naturally occurring pyrophosphate. Clodronate binds to calcium and inhibits osteoclastic bone resorption and hydroxyapatite crystal formation and dissolution, resulting in a reduction of bone turnover. This agent may control malignancy-associated hypercalcemia, inhibit osteolytic bone metastasis and decrease pain. A diphosphonate which affects calcium metabolism. It inhibits bone resorption and soft tissue calcification. |
| Molecular Formula |
CH2CL2NA2O6P2
|
|---|---|
| Molecular Weight |
288.86
|
| Exact Mass |
287.849
|
| CAS # |
22560-50-5
|
| Related CAS # |
Clodronate disodium tetrahydrate;88416-50-6;Clodronic acid;10596-23-3
|
| PubChem CID |
31195
|
| Appearance |
White to off-white solid powder
|
| Density |
2.306g/cm3
|
| Boiling Point |
474.7ºC at 760mmHg
|
| Melting Point |
>330ºC
|
| Flash Point |
240.9ºC
|
| Vapour Pressure |
2.55E-10mmHg at 25°C
|
| LogP |
1.307
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
13
|
| Complexity |
206
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
HJKBJIYDJLVSAO-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/CH4Cl2O6P2.2Na/c2-1(3,10(4,5)6)11(7,8)9;;/h(H2,4,5,6)(H2,7,8,9);;/q;2*+1/p-2
|
| Chemical Name |
disodium;[dichloro-[hydroxy(oxido)phosphoryl]methyl]-hydroxyphosphinate
|
| Synonyms |
clodronic acid disodium salt; Dichloromethylenediphosphonic acid disodium salt; Clodronate sodium; disodium dichloromethylene diphosphonate; Foreign brand names: Bonefos; Clasteon; Difosfonal; Loron; Mebonat; Ossiten. Code name: CL2MDP; DMDP.
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~100 mg/mL (~346.19 mM)
DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (346.19 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4619 mL | 17.3094 mL | 34.6188 mL | |
| 5 mM | 0.6924 mL | 3.4619 mL | 6.9238 mL | |
| 10 mM | 0.3462 mL | 1.7309 mL | 3.4619 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Bisfosfonatbehandling av bentransplantat vid höftrevisionhöftplastikrevision utvärderat med radiostereofotogrametri. En randomiserad, dubbel-blind studie hos patienter opererade för osteolys och aseptisk lossning.
CTID: null
Phase: Phase 2 Status: Completed
Date: 2007-04-25
Products are for research use only; We do not sell to patients
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