| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Toll-like receptor 7 (TLR7). CL264 is a TLR7-specific agonist that activates downstream signaling pathways, leading to the production of type I interferons and pro-inflammatory cytokines. This makes it a valuable tool for studying innate immune responses.
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| ln Vitro |
The most thoroughly researched class of receptors are toll-like receptors (TLRs), which are crucial for identifying a variety of base-, protein-, and sequence-based PAMPs. The majority of plasmacytoid dendritic cells (pDC) express TLR7. TLR7 modulators include adjuvants for vaccines, interferon inducers, anticancer, anti-infectious, and immune replacements [1].
CL264 is a TLR7-specific agonist that activates innate immune signaling pathways. By binding to TLR7, the compound triggers the production of type I interferons and pro-inflammatory cytokines. These activities are essential for understanding the role of TLR7 in immune responses. |
| ln Vivo |
In vivo activity of CL264 is expected to involve the activation of TLR7-mediated immune responses, leading to the production of interferons and cytokines. However, specific in vivo data are not extensively reported in the available literature. The compound is primarily used for in vitro immune signaling studies.
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| Enzyme Assay |
Receptor binding assays are performed using cells expressing TLR7. CL264 is incubated with the cells, and the activation of downstream signaling pathways is measured. The specificity for TLR7 over other TLRs is confirmed using appropriate controls.
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| Cell Assay |
Cell-based assays are conducted using immune cells such as dendritic cells or macrophages. Cells are treated with CL264, and the production of type I interferons and pro-inflammatory cytokines is measured. The activation of downstream signaling pathways, such as NF-κB and IRF, is assessed.
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| Animal Protocol |
In vivo studies for CL264 would involve the administration of the compound to animal models to evaluate TLR7-mediated immune activation. However, detailed in vivo data are not extensively reported in the available literature. The compound is primarily used for in vitro research.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of CL264 are not extensively reported in the available literature. The compound is soluble in DMSO (≥20 mg/mL) and is typically used in in vitro settings for immune signaling studies.
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| Toxicity/Toxicokinetics |
Toxicity data for CL264 are not extensively detailed in the available literature. The compound is intended for research use only. Its use as a TLR7 agonist suggests potential immunostimulatory effects that would need to be carefully evaluated in vivo.
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| References | |
| Additional Infomation |
CL264 is a research compound used to study innate immune signaling pathways, particularly TLR7-mediated immune responses. It is a valuable tool for understanding the role of TLR7 in infection, inflammation, and vaccine development. The compound is not approved for clinical use.
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| Molecular Formula |
C₁₉H₂₃N₇O₄
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|---|---|
| Molecular Weight |
413.43
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| Exact Mass |
413.181
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| CAS # |
1510712-69-2
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| PubChem CID |
90362358
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| Appearance |
Brown to gray solid powder
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| Density |
1.427±0.06 g/cm3(Predicted)
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| LogP |
0.9
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
30
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| Complexity |
626
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCNC1=NC(=C2C(=N1)N(C(=O)N2)CC3=CC=C(C=C3)C(=O)NCC(=O)O)N
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| InChi Key |
BXJWQQWEBUICHY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H23N7O4/c1-2-3-8-21-18-24-15(20)14-16(25-18)26(19(30)23-14)10-11-4-6-12(7-5-11)17(29)22-9-13(27)28/h4-7H,2-3,8-10H2,1H3,(H,22,29)(H,23,30)(H,27,28)(H3,20,21,24,25)
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| Chemical Name |
2-(4-((6-amino-2-(butylamino)-8-hydroxy-9H_purin_9-yl)methyl)benzamido)acetic acid
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| Synonyms |
CL264 CL-264 CL 264
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~60.47 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4188 mL | 12.0939 mL | 24.1879 mL | |
| 5 mM | 0.4838 mL | 2.4188 mL | 4.8376 mL | |
| 10 mM | 0.2419 mL | 1.2094 mL | 2.4188 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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