| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
| ln Vitro |
After three days of exposure, cinnamic acid (CINN) treatment of different tumor cells with epithelial and neuroectodermal origins led to dose-dependent growth suppression. The range of 1.2 to 4.5 mM is the inhibitory concentration (IC50) that causes a 50% decrease in the growth of tumor cells. Additionally, it was demonstrated that FS4 cells require 20 mM of cinnamic acid to produce an IC50 that is 5–20 times higher than that of tumor cells. Cinnamic acid not only prevents the growth of tumor cells but also causes morphological alterations that are compatible with the development of melanocytic cells. After being treated with 5 mM cinnamic acid for 5 days, melanoma 1011 cells showed signs of enlargement, a considerable rise in the cytoplasm to nucleus ratio, well-organized cytoskeleton, the formation of long dendritic processes, and high levels of melanization. Using a modified Boyden chamber with Matrigel-coated filters, an in vitro invasion experiment was used to evaluate changes in the capacity of melanoma 1011, A375 (M), and SKMEL28 cells to breakdown and penetrate tissue barriers after being treated with cinnamic acid. The three examined tumor cell lines showed a dose-dependent reduction of invasive capacity following three days of continuous cinnamic acid therapy. There is a 75–95% reduction in invasiveness after 5 mM cinnamic acid treatment [1].
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| References | |
| Additional Infomation |
Cinnamic acid is a monocarboxylic acid composed of acrylic acid with a phenyl substituent at the 3-position. It is found in cinnamon (Cinnamomum cassia) and is a plant metabolite. It belongs to the styrene class and the cinnamic acid class of compounds, and is also the conjugate acid of cinnamic esters. Trans-cinnamic acid is a metabolite found or produced by Escherichia coli (K12 strain, MG1655 strain). Cinnamic acid has been reported to exist in morning glory (Ipomoea leptophylla), tea tree (Camellia sinensis), and other organisms with relevant data. The molecular formula of cinnamic acid is C6H5CHCHCOOH. It is an odorless, white crystalline acid, slightly soluble in water. Its melting point is 133°C and its boiling point is 300°C. Cinnamic acid is a metabolite of Saccharomyces cerevisiae, produced or discovered by the yeast. See also: Cinnamon (one of the ingredients); Sodium cinnamate (active ingredient); Chinese cinnamon (one of the ingredients)... See more...
|
| Molecular Formula |
C9H8O2
|
|---|---|
| Molecular Weight |
148.1586
|
| Exact Mass |
148.052
|
| CAS # |
621-82-9
|
| PubChem CID |
444539
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
265.0±0.0 °C at 760 mmHg
|
| Melting Point |
133ºC
|
| Flash Point |
189.5±9.6 °C
|
| Vapour Pressure |
0.0±0.5 mmHg at 25°C
|
| Index of Refraction |
1.616
|
| LogP |
2.41
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
11
|
| Complexity |
155
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C(C=C1)/C=C/C(=O)O
|
| InChi Key |
WBYWAXJHAXSJNI-VOTSOKGWSA-N
|
| InChi Code |
InChI=1S/C9H8O2/c10-9(11)7-6-8-4-2-1-3-5-8/h1-7H,(H,10,11)/b7-6+
|
| Chemical Name |
(E)-3-phenylprop-2-enoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Ethanol :≥ 50 mg/mL (~337.47 mM)
DMSO : ~50 mg/mL (~337.47 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.87 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.87 mM) (saturation unknown) in 10% EtOH + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (16.87 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7495 mL | 33.7473 mL | 67.4946 mL | |
| 5 mM | 1.3499 mL | 6.7495 mL | 13.4989 mL | |
| 10 mM | 0.6749 mL | 3.3747 mL | 6.7495 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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