| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
|
| ln Vitro |
After SO2 model treatment, CHPG sodium salt (10-500 μM; 24 hours) dramatically enhanced cell survival and reduced LDH release [1]. CHPG sodium salt (0.5 mM; 30 minutes) protects the BV2 cell scaffold SO2 cell model. Treatment with CHPG sodium salt (0.5 mM; 30 minutes) alone increases the expression of TSG-6 at the mRNA and protein levels [1].
|
|---|---|
| Cell Assay |
Cell Viability Assay[1]
Cell Types: BV2 microglial cells Tested Concentrations: 10, 50, 100 and 500 µM Incubation Duration: 24 hrs (hours) Experimental Results: Increased cell viability. Apoptosis analysis[1] Cell Types: BV2 microglia Tested Concentrations: 0.5 mM Incubation Duration: 30 minutes Experimental Results: Protection of BV2 cells from SO2-induced apoptosis. Western Blot Analysis[1] Cell Types: BV2 Microglia Tested Concentrations: 0.5 mM Incubation Duration: 30 minutes Experimental Results: TSG-6 mRNA and protein level expression increased. |
| Animal Protocol |
Animal/Disease Models: Adult SD (SD (Sprague-Dawley)) male rats, body weight 280-320 g[2]
Doses: 250 nM Route of Administration: injection; CHPG sodium salt (injection; 250 nM; 7 days) Dramatically reduces the volume of brain damage[2 ]. Results for 7 days: The size of the brain lesions was Dramatically diminished. |
| References |
|
| Molecular Formula |
C8H7CLNNAO3
|
|---|---|
| Molecular Weight |
223.58885216713
|
| Exact Mass |
223.001
|
| CAS # |
1303993-73-8
|
| Related CAS # |
CHPG;170846-74-9
|
| PubChem CID |
52974246
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
14
|
| Complexity |
205
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
KZRZZIISUUINJT-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C8H8ClNO3.Na/c9-6-2-1-4(11)3-5(6)7(10)8(12)13;/h1-3,7,11H,10H2,(H,12,13);/q;+1/p-1
|
| Chemical Name |
sodium;2-amino-2-(2-chloro-5-hydroxyphenyl)acetate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~447.25 mM)
H2O : ~6.67 mg/mL (~29.83 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.18 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.18 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.18 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 5 mg/mL (22.36 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4725 mL | 22.3624 mL | 44.7247 mL | |
| 5 mM | 0.8945 mL | 4.4725 mL | 8.9449 mL | |
| 10 mM | 0.4472 mL | 2.2362 mL | 4.4725 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
