| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
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| Targets |
Chlormidazole HCl targets fungal and bacterial cells through its azole antifungal mechanism. As an imidazole derivative, it inhibits the synthesis of ergosterol, a key component of fungal cell membranes. This disruption of membrane integrity leads to fungal cell death. The compound also has activity against some Gram-positive cocci, indicating a broader antimicrobial spectrum. Its mechanism of action is characteristic of azole antifungals, which are widely used in the treatment of fungal infections.
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| ln Vitro |
Chlormidazole HCl exhibits in vitro antifungal activity against a variety of fungi. It also has inhibitory activity against some Gram-positive cocci. The compound’s activity is assessed using standard broth microdilution or agar diffusion methods to determine minimum inhibitory concentrations (MICs) against fungal and bacterial strains. These in vitro activities confirm its potential as an antifungal and antibacterial agent. The compound is used in dermatological research for the study of fungal and bacterial infections of the skin and nails.
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| ln Vivo |
Chlormidazole HCl is used in vivo as an antifungal and antibacterial agent. It is indicated for fungal and bacterial infections of the nails and skin, including interdigital and periungual mycoses. The compound is applied topically for the treatment of these infections. Its efficacy depends on the susceptibility of the infecting organism and the ability to achieve adequate drug concentrations at the site of infection. The compound’s use in dermatology has been documented.
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| Enzyme Assay |
In vitro antifungal assays for Chlormidazole HCl involve measuring its activity against various fungal strains. Standard broth microdilution or agar diffusion methods are employed to determine the minimum inhibitory concentration (MIC). Fungal cultures are grown in appropriate media, and serial dilutions of Chlormidazole HCl are added. After incubation, the MIC is determined as the lowest concentration that inhibits visible fungal growth. Antibacterial activity against Gram-positive cocci is assessed similarly.
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| Cell Assay |
In vitro cellular assays for Chlormidazole HCl are conducted in fungal and bacterial cultures. Fungal strains are cultured in appropriate medium and treated with varying concentrations of the compound. Fungal growth is monitored by optical density or by measuring metabolic activity. Cytotoxicity assays in mammalian cell lines may be performed to assess selectivity. These assays confirm the compound’s antifungal and antibacterial activity.
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| Animal Protocol |
In vivo animal experiments with Chlormidazole HCl are conducted in animal models of fungal and bacterial infections. Rodent models of dermatophyte infection or skin infection are commonly used. Animals are infected with a pathogenic fungal or bacterial strain, and Chlormidazole HCl is applied topically at varying doses. Efficacy endpoints include reduction in lesion size, fungal load, and clinical signs of infection. These studies define the compound’s in vivo efficacy.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Chlormidazole HCl are limited, as the compound is primarily used topically. It has a molecular weight of 293.19 and is soluble in organic solvents. When applied topically, systemic absorption is minimal. The compound’s half-life and distribution have not been extensively characterized. Further PK studies would be needed if the compound were to be developed for systemic use.
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| Toxicity/Toxicokinetics |
Chlormidazole HCl is generally well-tolerated when used topically. Common adverse effects may include local irritation or allergic reactions. Systemic toxicity is minimal due to limited absorption. The compound is contraindicated in patients with known hypersensitivity to azole antifungals. Standard safety precautions should be followed when handling the compound.
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| Additional Infomation |
Chlormidazole HCl is an antifungal imidazole derivative used for the treatment of fungal and bacterial infections of the nails and skin. It is also known as clomidazole hydrochloride, H-115, and Myco-polycid. The compound works by inhibiting ergosterol synthesis and disrupting fungal cell membranes. It has activity against a variety of fungi and some Gram-positive cocci. Chlormidazole HCl is available in high purity for research applications. Its use in dermatology and microbiology research is well-established.
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| Molecular Formula |
C15H14CL2N2
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|---|---|
| Molecular Weight |
293.1911
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| Exact Mass |
292.053
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| CAS # |
74298-63-8
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| PubChem CID |
11346885
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| Appearance |
Off-white to light yellow solid powder
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| Boiling Point |
467.5ºC at 760 mmHg
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| Flash Point |
236.5ºC
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| LogP |
4.848
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
19
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| Complexity |
276
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=NC2=CC=CC=C2N1CC3=CC=C(C=C3)Cl.Cl
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| InChi Key |
MHMTXDMLQZHXRZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H13ClN2.ClH/c1-11-17-14-4-2-3-5-15(14)18(11)10-12-6-8-13(16)9-7-12;/h2-9H,10H2,1H3;1H
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| Chemical Name |
1-[(4-chlorophenyl)methyl]-2-methylbenzimidazole;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~213.17 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4108 mL | 17.0538 mL | 34.1076 mL | |
| 5 mM | 0.6822 mL | 3.4108 mL | 6.8215 mL | |
| 10 mM | 0.3411 mL | 1.7054 mL | 3.4108 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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