| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
||
| Other Sizes |
Purity: ≥98%
| Targets |
Chicago Sky Blue 6B binds to the N‑terminal and NAC regions of α‑synuclein (KD = 0.64 μM) and inhibits α‑synuclein aggregation and cell‑to‑cell propagation[4]
|
|---|---|
| ln Vitro |
Chicago Sky Blue 6B stains fungal hyphae and spores of Malassezia blue against a light purplish background of cellular debris[1]
Chicago Sky Blue 6B staining showed positive results in 98 out of 100 skin scraping samples (98%) for pityriasis versicolor[1] The sensitivity of Chicago Sky Blue 6B stain was 100% using KOH mount as the reference method[1] The overall agreement between Chicago Sky Blue 6B stain and KOH mount was 94% (κ=0.38, P<0.001)[1] |
| ln Vivo |
Chicago Sky Blue 6B (20 mg/kg/day, i.p., once weekly for 3 months) improves forelimb grip strength in A53T α‑synuclein transgenic mice[4]
Chicago Sky Blue 6B improves motor coordination and reduces slips in balance beam tests in A53T transgenic mice[4] Chicago Sky Blue 6B reduces phosphorylated α‑synuclein (pS129) deposition in the hippocampus and prefrontal cortex[4] Chicago Sky Blue 6B reduces astrogliosis (GFAP immunoreactivity) in the prefrontal cortex[4] |
| Enzyme Assay |
Fluorescence polarization assay: NBD‑labeled PrPC was incubated with test compounds in FP buffer; Aβ oligomers were added, and FP signals were recorded to measure compound‑mediated inhibition of Aβ–PrP binding[3]
ELISA assay: PrP was coated onto microplates; compounds were added prior to biotinylated Aβ oligomers, and binding was detected using time‑resolved fluorescence to quantify inhibition[3] Isothermal titration calorimetry: PrP constructs in buffer were titrated with Chicago Sky Blue 6B at constant temperature, and thermodynamic parameters were recorded and fitted to a one‑site binding model[3] |
| Cell Assay |
Cell‑based Aβ binding assay: COS‑7 cells were transiently transfected to express full‑length mouse PrP; cells were incubated with biotinylated Aβ oligomers and graded concentrations of Chicago Sky Blue 6B, and bound Aβ was visualized and quantified[3]
Cell‑based anti‑prion assay: Chronically RML prion‑infected N2a PK1 cells were treated with Chicago Sky Blue 6B for 3 days; PrPSc levels were measured by dot blot after proteinase K digestion, and cytotoxicity was assessed in parallel[3] |
| Animal Protocol |
Six‑month‑old A53T α‑synuclein transgenic mice and wild‑type littermates were administered Chicago Sky Blue 6B by intraperitoneal injection at 20 mg/kg/day, once weekly for 3 months; control groups received PBS[4]
After treatment, mice underwent behavioral tests (open field, grip strength, balance beam) followed by perfusion, brain sectioning, and immunohistochemical analysis of pS129 α‑synuclein and GFAP[4] Six‑month‑old A53T α‑synuclein transgenic mice and wild‑type littermates were administered Chicago Sky Blue 6B by intraperitoneal injection at 20 mg/kg/day, once weekly for 3 months; control groups received PBS[4] After treatment, mice underwent behavioral tests (open field, grip strength, balance beam) followed by perfusion, brain sectioning, and immunohistochemical analysis of pS129 α‑synuclein and GFAP[4] |
| ADME/Pharmacokinetics |
Chicago Sky Blue 6B is highly water‑soluble and shows limited brain bioavailability[4]
|
| Toxicity/Toxicokinetics |
Interactions
Chicago Sky Blue (also known as Niagara Sky Blue) is an important dye that has been successfully used as a target for intravascular energy absorption in helium-neon lasers. This light/dye interaction leads to endothelial damage, the extent of which can be controlled by adjusting the laser irradiation time and the amount of dye injected intravenously. Endothelial damage is likely due to the heat generated by the dye absorbing energy at the plasma-endothelial cell interface. The mildest damage results in the selective loss of the vasodilatory effects normally produced by acetylcholine and bradykinin (two endothelium-dependent vasodilators). The vasodilatory effects produced by sodium nitroprusside (a vasodilator that acts directly on vascular smooth muscle) are preserved. More severe damage (e.g., longer light exposure time and/or more dye) can lead to localized platelet aggregation at the laser irradiation site. Chicago Sky Blue 6B dye is reported to be corrosive on contact; low concentrations used in this study are unlikely to cause significant adverse effects[1] |
| References |
|
| Additional Infomation |
Direct Blue 1 is a bright green solid or a dark blue powder. (NTP, 1992)
Chicago Sky Blue 6B is a direct disazo dye compound mainly used in the textile industry for cotton dyeing[1] Chicago Sky Blue 6B can be used as a counterstain for background autofluorescence in immunofluorescence histochemistry[1] Chicago Sky Blue 6B stain is prepared as a 1% solution by dissolving the dye in distilled water[1] Chicago Sky Blue 6B is used with 10% KOH as a clearing agent for microscopic examination[1] Chicago Sky Blue 6B stain enables clear visualization of fungal morphology even under low‑power microscope objectives[1] Chicago Sky Blue 6B stain is a rapid, sensitive, cost‑effective contrast stain for the diagnosis of pityriasis versicolor[1] |
| Molecular Formula |
C34H24N6NA4O16S4
|
|---|---|
| Molecular Weight |
992.8040
|
| Exact Mass |
991.972
|
| CAS # |
2610-05-1
|
| PubChem CID |
17460
|
| Appearance |
Light blue to blue solid powder
|
| LogP |
10.185
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
22
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
64
|
| Complexity |
1830
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
BPHHNXJPFPEJOF-UHFFFAOYSA-J
|
| InChi Code |
InChI=1S/C34H28N6O16S4.4Na/c1-55-23-11-15(3-7-19(23)37-39-21-9-5-17-25(57(43,44)45)13-27(59(49,50)51)31(35)29(17)33(21)41)16-4-8-20(24(12-16)56-2)38-40-22-10-6-18-26(58(46,47)48)14-28(60(52,53)54)32(36)30(18)34(22)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
|
| Chemical Name |
tetrasodium;4-amino-6-[[4-[4-[(8-amino-1-hydroxy-5,7-disulfonatonaphthalen-2-yl)diazenyl]-3-methoxyphenyl]-2-methoxyphenyl]diazenyl]-5-hydroxynaphthalene-1,3-disulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~125.91 mM)
H2O : ~2.08 mg/mL (~2.10 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 4.17 mg/mL (4.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 41.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 4.17 mg/mL (4.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 41.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 5 mg/mL (5.04 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0073 mL | 5.0363 mL | 10.0725 mL | |
| 5 mM | 0.2015 mL | 1.0073 mL | 2.0145 mL | |
| 10 mM | 0.1007 mL | 0.5036 mL | 1.0073 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA