| Size | Price | Stock | Qty |
|---|---|---|---|
| 50g |
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| Other Sizes |
| Targets |
CHES does not have a specific biological target as a drug. It is a zwitterionic buffer used to maintain pH in biochemical and enzymatic assays. It has been shown to bind to the iodoacetate binding site of liver alcohol dehydrogenase with high affinity. It can also interact with hemagglutinin (HA) and mimic sialic acid binding. Good's buffers are characterized by high water solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability.
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|---|---|
| ln Vitro |
CHES does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for studying pH-dependent processes in enzymology and biochemistry. The compound maintains a stable pH environment for enzymatic reactions without interfering with the biochemical processes being studied. It has minimal absorption in the UV-visible spectral range, which is advantageous during spectrophotometric analysis.
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| ln Vivo |
CHES does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro applications.
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| Enzyme Assay |
In vitro enzyme assays using CHES as a buffer are performed to study pH-dependent enzymatic processes. The buffer is prepared at the desired pH within its effective range (8.6-10.0). Enzyme activity is measured under controlled pH conditions, and the effects of pH on enzyme kinetics, stability, and substrate binding are evaluated. CHES has been shown to have high affinity for the iodoacetate binding site of liver alcohol dehydrogenase.
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| Cell Assay |
Cell culture experiments using CHES are limited as it is primarily used as a buffer in biochemical assays rather than in cell culture media. Good's buffers like CHES are designed for minimal interference with biochemical processes and have low cell membrane permeability. The compound may be used in cell-free assays or in preparing buffers for cell lysis and protein extraction. Researchers should consult primary literature for specific applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to CHES as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research. Researchers should not attempt in vivo administration of this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to CHES as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals or humans. Its physicochemical properties include molecular weight 207.29, molecular formula C8H17NO3S, and pKa 9.5. It is highly water-soluble. These properties are relevant for buffer preparation rather than pharmacokinetics.
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| Toxicity/Toxicokinetics |
CHES is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use.
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| References | |
| Additional Infomation |
N-Cyclohexyl-2-aminoethanesulfonic acid is an alkylsulfonic acid, a derivative of cyclohexylamine, in which one amino hydrogen atom is replaced by a 2-sulfoethyl group. It is used as a buffer (pH range 8.6–10.0) in enzymatic studies of pH-dependent processes. It is a Goodyear buffer. It is an alkylsulfonic acid and a secondary fatty amine.
See also: Beef extract (note moved to). CHES has CAS number 103-47-9, molecular formula C8H17NO3S, and molecular weight 207.29. Synonyms: 2-(Cyclohexylamino)ethanesulfonic acid, N-cyclohexyltaurine. It is a zwitterionic Good's buffer with pH range 8.6-10.0 and pKa 9.5. Purity: typically ≥99%. Storage: room temperature. Not for human use; for research purposes only. |
| Molecular Formula |
C8H17NO3S
|
|---|---|
| Molecular Weight |
207.2905
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| Exact Mass |
207.092
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| CAS # |
103-47-9
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| Related CAS # |
134737-05-6;3076-05-9 (mono-hydrochloride salt)
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| PubChem CID |
66898
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Melting Point |
≥300 °C
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| Index of Refraction |
1.519
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| LogP |
-0.17
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
13
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| Complexity |
226
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MKWKNSIESPFAQN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)
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| Chemical Name |
2-(cyclohexylamino)ethanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~166.67 mg/mL (~804.04 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (482.42 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8242 mL | 24.1208 mL | 48.2416 mL | |
| 5 mM | 0.9648 mL | 4.8242 mL | 9.6483 mL | |
| 10 mM | 0.4824 mL | 2.4121 mL | 4.8242 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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