| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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Purity: ≥98%
| Targets |
Fluorescent dye
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|---|---|
| ln Vitro |
CellTrack Blue CMAC (Known as CellTracker™ Blue CMAC, Trade Mark of Molecular Probes) is a fluorescent thiol-reactive cell permeant probe that easily pass through cell membranes. Upon reacting with thio-containing compound, the probe is well retained in cells and is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with this probe are typically fluorescent and viable for at least 24 hours, making these probes excellent long-term cell tracers. Furthermore, this probe can be fixed with formaldehyde or glutaraldehyde to allow long-term storage of the labeled cells or tissue, to facilitate safe handling of potentially pathogenic samples.
Advantages of CellTracker Blue CMAC: 1) Easy to use - remove culture medium, add dye, incubate for 30 minutes, perform cell imaging 2) Fluorescence signal retention>72 hours (usually 3 to 6 generations) 3) Blue excitation/emission spectrum suitable for multi-channel technology (maximum value of 353/466 nm) 4) Low cytotoxicity - does not affect viability or proliferation |
| ln Vivo |
An inclusive chemical definition of “reactive” dyeing of textiles is introduced, encompassing the CI Azoic, CI Mordant, CI Reactive, CI Sulphur and CI Vat dye application classes. Such reactive dyeing increases fibre retention of dye and makes application practically possible. The analogous application of dyes and fluorescent probes as microscopic stains in biology and medicine is outlined, focussing on using reactive fluorescent probes with living cells. Parallels with textile dyeing are noted, eg, enhanced probe retention and facilitation of probe application. However, the primary purpose of using reactive probes with live cells is detection of properties of biological systems: to identify biological structures and chemical/biochemical contents; assess biological functions and physicochemical properties; and determine changes in locations of cells and cell components. Problems occurring with such probes are outlined, particularly the problematic character of many standard protocols, and localisation artefacts arising with reactive probes whose reactant and product species are physiochemically significantly different. This latter problem is explored via a case study of possible reactant/product artefacts with probes for reactive oxygen species. Comparison of experimental observations of probe localisations with the localisations predicted using quantitative structure activity (QSAR) modelling indicates that such artefacts can occur with a significant proportion of chemically diverse, widely used, commercially available probes, as well as with experimental compounds reported in the literature. A graphical flowchart is provided to assess possible occurrence of reactant/product artefacts arising with reactive fluorescent probes localising in various organelles of living cells [1].
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| Enzyme Assay |
CellTracker Blue CMAC Dye is a fluorescent dye that is very suitable for monitoring cell movement or position. CellTracker Blue CMAC fluorescent dye can freely pass through the cell membrane and enter the cell, and is converted into a reactive product that does not penetrate the cell membrane within the cell. This dye can be transferred to daughter cells, but it will not transfer to adjacent cells in the population. The CellTracker Blue CMAC dye is designed to display fluorescence for at least 72 hours and has ideal tracking properties: stable at working concentrations, non-toxic, well retained in cells, and emitting bright fluorescence at physiological pH values. In addition, The excitation and emission spectra of CellTracker Blue CMAC dye are completely separated from GFP (green fluorescent protein) and RFP (red fluorescent protein) spectra and can be multiplexed.
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| Cell Assay |
Experimental procedure:
1. Dissolve the CMAC solid in anhydrous DMSO and prepare a 10 mM stock solution. Dilute the stock solution to a working solution of 0.5-25 μ M using serum-free culture medium. Preheat the working fluid at 37 ℃ before use 2. Incubate for 15-45 minutes under different cell culture conditions according to different cell types. For suspended cells, centrifuge the supernatant and collect cell precipitates. Resuspend cells with preheated staining solution; For adherent cells, when the cell grows to the appropriate cell density, remove the supernatant culture medium and add preheated staining solution 3. Remove the staining solution, add fresh preheated cell culture medium, and incubate at 37 ℃ for 30 minutes 4. For suspended cells, draw about 5 μ l of stained cells and drop them onto a glass slide. Cover the slide with a cover glass and observe under a fluorescence microscope; For adherent cells, direct observation is sufficient. (Optional) If you need to fix the cells, you can skip step 4 first and prepare for observation after the fixation step is completed 5. Use PBS to wash cells 6. Fix cells with PBS containing 3.7% paraformaldehyde and incubate at room temperature for 15 minutes 7. Use PBS to wash cells 8. If necessary, the cell membrane can be permeabilized. When cells need to be labeled with antibodies, membrane permeabilization is necessary to facilitate antigen entry into the cells. Cold acetone can be used to treat cells for 10 minutes, which can provide cell permeability. |
| References |
| Molecular Formula |
C10H8NO2CL
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|---|---|
| Molecular Weight |
209.62902
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| Exact Mass |
209.024
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| CAS # |
147963-22-2
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| PubChem CID |
2762598
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| Appearance |
White to off-white solid powder
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| Density |
1.399 g/cm3
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| Boiling Point |
410.3ºC at 760 mmHg
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| Flash Point |
201.9ºC
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| Vapour Pressure |
6.1E-07mmHg at 25°C
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| Index of Refraction |
1.633
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| LogP |
2.695
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
14
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| Complexity |
277
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC2=C(C=C1N)OC(=O)C=C2CCl
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| InChi Key |
VIEYMVWPECAOCY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H8ClNO2/c11-5-6-3-10(13)14-9-4-7(12)1-2-8(6)9/h1-4H,5,12H2
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| Chemical Name |
7-amino-4-(chloromethyl)chromen-2-one; 7-AMINO-4-CHLOROMETHYLCOUMARIN
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| Synonyms |
7-amino-4-chloromethylcoumarin; 7-amino-4-(chloromethyl)-2H-chromen-2-one; 7-amino-4-(chloromethyl)chromen-2-one; CellTracker Blue CMAC; 147963-22-2; 7-amino-4-(chloromethyl)-2H-chromen-2-one; 7-amino-4-(chloromethyl)chromen-2-one; 2H-1-Benzopyran-2-one, 7-amino-4-(chloromethyl)-; CMAC cpd; CMAC; .
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~596.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (9.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7703 mL | 23.8515 mL | 47.7031 mL | |
| 5 mM | 0.9541 mL | 4.7703 mL | 9.5406 mL | |
| 10 mM | 0.4770 mL | 2.3852 mL | 4.7703 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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