| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Targets |
CB7921220 targets adenylyl cyclase (AC) isoforms AC1 and AC6. Adenylyl cyclase is an enzyme that converts ATP to cyclic AMP (cAMP), an important second messenger in many biological processes. By inhibiting AC1 and AC6, CB7921220 reduces cAMP production and modulates downstream signaling pathways. It shows no effect on AC2 and AC5.
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| ln Vitro |
Adenylyl cyclase (AC) 1 has some isoform selectivity in CB-7921220, yet it is unable to distinguish between AC1 and AC6. The lack of selectivity of CB-7921220 between AC1 and AC6 may be explained by its more consistently predicted binding position in the two virtual docking screens and similar binding conformation to ATP and P-site inhibitors [1].
CB7921220 is a selective inhibitor of adenylyl cyclase isoforms AC1 and AC6. At a concentration of 100 μM, it reduces AC1 and AC6 activity by 60% in Sf9 membranes stimulated by forskolin. It has no effect on AC2 and AC5, demonstrating isoform selectivity. |
| ln Vivo |
Specific in vivo activity data for CB7921220 is not extensively detailed in the available literature. As a research compound, its in vivo effects are primarily studied in the context of AC1 and AC6 inhibition and its impact on cAMP-mediated signaling pathways. Further studies are needed to fully characterize its in vivo efficacy and pharmacokinetic properties.
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| Enzyme Assay |
CB7921220's activity as an AC inhibitor is assessed using in vitro enzyme assays that measure its ability to inhibit adenylyl cyclase activity. These assays typically involve measuring cAMP production in cell membranes or purified enzyme preparations. The compound's selectivity for AC1 and AC6 over AC2 and AC5 is evaluated.
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| Cell Assay |
Cellular assays for CB7921220 involve treating cells with the compound and measuring cAMP levels and downstream signaling. The compound's ability to inhibit AC1 and AC6-mediated cAMP production is assessed. Its effects on cellular processes regulated by cAMP, such as gene expression and cell function, are evaluated.
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| Animal Protocol |
In vivo animal model protocols for CB7921220 would involve its administration to animal models to study the role of AC1 and AC6 in various physiological and pathological processes. The compound's effects on cAMP signaling, behavior, and disease phenotypes would be assessed.
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| ADME/Pharmacokinetics |
CB7921220 has a molecular weight of 240.26 and a molecular formula of C14H12N2O2. It is supplied as a powder or in solution. Specific pharmacokinetic data is not extensively detailed in the available literature. As a small molecule inhibitor, its pharmacokinetic properties would need to be evaluated in preclinical studies.
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| Toxicity/Toxicokinetics |
CB7921220 is intended for research use only and not for human consumption. As a research compound, its comprehensive toxicological profile may not be fully characterized. Standard safety assessments would be required for clinical development.
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| References | |
| Additional Infomation |
CB7921220 (CAS# 115453-99-1) is a research compound used to study adenylyl cyclase function and cAMP signaling. It is a selective inhibitor of AC1 and AC6. It is not approved for clinical use and is supplied as a research chemical.
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| Molecular Formula |
C14H12N2O2
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|---|---|
| Molecular Weight |
240.257283210754
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| Exact Mass |
240.09
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| CAS # |
115453-99-1
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| PubChem CID |
5740547
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| Appearance |
Pink to red solid powder
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| Melting Point |
225 - 226 °C
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
309
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=NC(=C1)C(=O)O)/C=C/C2=CC=C(C=C2)N
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~208.11 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1622 mL | 20.8108 mL | 41.6216 mL | |
| 5 mM | 0.8324 mL | 4.1622 mL | 8.3243 mL | |
| 10 mM | 0.4162 mL | 2.0811 mL | 4.1622 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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