| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
CAPS does not have a specific biological target as a drug. It is a zwitterionic buffer used to maintain pH in biochemical and biological experiments. Good's buffers are characterized by high water solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability. CAPS is used in various applications including Western blotting, protein sequencing, and electrophoresis. It is also used in the electrotransfer of proteins to membranes and in HPLC separation.
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|---|---|
| ln Vitro |
Because amines do not ionize at high pH values, CAPS surfactants have a single negative charge [1].
CAPS does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and biological research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications including protein electrophoresis, Western blotting, and enzyme assays. It is also used in the preparation of transfer buffers for protein blotting. |
| ln Vivo |
CAPS does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications. It is not administered in vivo.
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| Enzyme Assay |
In vitro biochemical assays using CAPS buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH within its effective range (7.9-11.1). CAPS buffer is used in Western blotting and immunoblotting experiments, as well as in protein sequencing and identification. It is used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. It is also used in biochemical diagnostics, DNA/RNA extraction, and PCR diagnostics.
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| Cell Assay |
Cell culture experiments using CAPS are limited as it is primarily used as a buffer in biochemical assays rather than in cell culture media. The buffer's pH range (7.9-11.1) is above physiological pH, making it unsuitable for most cell culture applications. It may be used in cell lysis buffers or in protein extraction protocols. Researchers should consult primary literature for specific applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to CAPS as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research. Researchers should not attempt in vivo administration of this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to CAPS as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals or humans. Its physicochemical properties include a molecular weight of 221.32, a molecular formula of C9H19NO3S, a pKa of 10.0-10.6, and a useful pH range of 7.9-11.1. It is highly water-soluble. Storage: room temperature, protected from moisture.
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| Toxicity/Toxicokinetics |
CAPS is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use. No specific toxicity data are available.
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| References |
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| Additional Infomation |
Cyclohexylaminopropanesulfonic acid is an amine.
CAPS has CAS number 1135-40-6, molecular formula C9H19NO3S, and molecular weight 221.32. It is a zwitterionic Good's buffer with a pH range of 7.9-11.1 and a pKa of 10.0-10.6 at 25°C. It is widely used in Western blotting, protein sequencing, and electrotransfer of proteins to membranes. Purity: typically ≥99%. Not for human use; for research purposes only. |
| Molecular Formula |
C9H19NO3S
|
|---|---|
| Molecular Weight |
221.32
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| Exact Mass |
259.085
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| CAS # |
1135-40-6
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| Related CAS # |
3-(Cyclohexylamino)-1-propanesulfonic Acid-d17;1219804-15-5
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| PubChem CID |
70815
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Melting Point |
>300 °C(lit.)
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| Index of Refraction |
1.515
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| LogP |
0.05
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
14
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| Complexity |
239
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C([H])([H])C([H])([H])C([H])([H])N([H])C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C1([H])[H])(=O)(=O)O[H]
|
| InChi Key |
PJWWRFATQTVXHA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H19NO3S/c11-14(12,13)8-4-7-10-9-5-2-1-3-6-9/h9-10H,1-8H2,(H,11,12,13)
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| Chemical Name |
3-(cyclohexylamino)propane-1-sulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~564.79 mM)
DMSO : ~5 mg/mL (~22.59 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.5 mg/mL (2.26 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.5 mg/mL (2.26 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5183 mL | 22.5917 mL | 45.1834 mL | |
| 5 mM | 0.9037 mL | 4.5183 mL | 9.0367 mL | |
| 10 mM | 0.4518 mL | 2.2592 mL | 4.5183 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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