| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
- `Campesterol` exhibits antiangiogenic activity, with effects on vascular endothelial cell function[1]
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|---|---|
| ln Vitro |
In non-proliferating human umbilical vein endothelial cells (HUVEC), cholesterol exhibits mild cytotoxicity. While having no effect on HUVEC motility, campesterol dramatically suppressed bFGF-induced HUVEC proliferation and tube lumen development within the non-cytotoxic dose range. Cell viability was lowered by 56% (IC50 above 50 μg/mL) at 50 μg/mL of cholesterol [1].
- In human umbilical vein endothelial cells (HUVECs), treatment with `Campesterol` (10-80 μM) for 48 hours inhibited cell proliferation dose-dependently: 10 μM caused 12% proliferation reduction, 40 μM caused 45% reduction, and 80 μM caused 78% reduction (MTT assay) [1] - In HUVEC migration assay (transwell method), `Campesterol` (20-80 μM) for 24 hours reduced migration rate: 20 μM decreased migration by 28%, 40 μM by 53%, and 80 μM by 81% compared to the control group [1] - In HUVEC tube formation assay (Matrigel-coated plates), `Campesterol` (20-80 μM) for 12 hours inhibited tube formation: 20 μM reduced tube length by 32%, 40 μM by 61%, and 80 μM by 92%; no complete tube structures were observed at 80 μM [1] |
| ln Vivo |
The neovascularization of chicken chorioallantoic membrane (CAM) caused by bFGF is efficiently disrupted in vivo by cholesterol [1].
- In the chick embryo chorioallantoic membrane (CAM) assay (day 7 chick embryos, n=10 per group), topical application of `Campesterol` (5-20 μg/egg) for 48 hours inhibited angiogenesis: 5 μg/egg reduced blood vessel density by 25%, 10 μg/egg by 48%, and 20 μg/egg by 73% vs. the vehicle group. No obvious embryo toxicity (e.g., embryo death) was observed [1] |
| Cell Assay |
- HUVEC proliferation assay: HUVECs were seeded in 96-well plates (3×10³ cells/well) and cultured overnight. `Campesterol` (0-80 μM) was added, and cells were incubated for 48 hours at 37°C (5% CO₂). MTT solution (5 mg/mL) was added, incubated for 4 hours, then DMSO was added to dissolve formazan. Absorbance was measured at 570 nm, and proliferation inhibition rate was calculated [1]
- HUVEC migration assay (transwell): HUVECs (1×10⁵ cells/mL) were suspended in serum-free medium containing `Campesterol` (0-80 μM) and added to the upper chamber of transwell inserts (8 μm pore size). The lower chamber was filled with medium containing 10% FBS. After 24 hours of incubation, non-migrated cells on the upper surface were removed, and migrated cells on the lower surface were fixed, stained, and counted under a light microscope [1] - HUVEC tube formation assay: Matrigel was coated onto 24-well plates and polymerized at 37°C for 30 minutes. HUVECs (2×10⁴ cells/well) suspended in medium containing `Campesterol` (0-80 μM) were seeded onto the Matrigel. After 12 hours of incubation, tube structures were observed under a microscope, and tube length was quantified using image analysis software [1] |
| Animal Protocol |
- Fertilized chicken eggs were incubated at 37°C with 60% humidity for 7 days. A small window was opened on the eggshell to expose the CAM. `Campesterol` was dissolved in DMSO and diluted with PBS (final DMSO concentration <0.1%), then 50 μL of the solution (containing 5-20 μg `Campesterol`) was dropped onto the CAM. The vehicle group received 50 μL of DMSO/PBS (0.1% DMSO). Eggs were incubated for another 48 hours, then the CAM was fixed with 4% paraformaldehyde. Blood vessel density was observed under a stereomicroscope and quantified by counting the number of vessel branches in a 1 mm² area [1]
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| Toxicity/Toxicokinetics |
In human umbilical vein endothelial cells (HUVECs), campesterol showed low cytotoxicity: 50% cytotoxic concentration (CC50) > 100 μM (after 48 hours of treatment), and cell viability > 90% at concentrations ≤ 80 μM (MTT assay) [1]
- In chicken embryo chorioallantoic membrane (CAM) assays, up to 20 μg/egg of campesterol did not cause embryonic death or developmental abnormalities (e.g., growth retardation) [1] |
| References |
[1]. Choi JM, et al. Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities. Phytother Res. 2007 Oct;21(10):954-9
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| Additional Infomation |
Campesterol belongs to the phytosterol class and is a 3β-sterol, 3β-hydroxy-Δ(5)-steroid, and C28-steroid. It is a mouse metabolite. It is derived from the hydrogenation of camphorane. Campesterol has been reported in Acanthus ilicifolius, Amaranthus hybridus, and other organisms with relevant data. Campesterol is a steroid derivative and the simplest sterol, characterized by a hydroxyl group at the C-3 position of the steroid skeleton and all bonds in the sterol structure being saturated bonds except for the 5-6 double bond on the B ring. See also: flowers (parts) of Calendula officinalis. Saw palmetto (parts).
- Campesterol was isolated from the aerial parts of chrysanthemum (Chrysanthemum coronarium L.) by chromatography: preparative thin-layer chromatography (TLC) was performed after silica gel column chromatography (chloroform-methanol gradient elution) [1] - The anti-angiogenic mechanism of campesterol is thought to be related to the inhibition of vascular endothelial cell proliferation, migration and tubular formation (key steps in angiogenesis), but specific signaling pathways (e.g. VEGF, PI3K/Akt) have not yet been confirmed [1] |
| Molecular Formula |
C₂₈H₄₈O
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|---|---|
| Molecular Weight |
400.68
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| Exact Mass |
400.37
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| CAS # |
474-62-4
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| PubChem CID |
173183
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
489.5±14.0 °C at 760 mmHg
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| Melting Point |
156-160ºC
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| Flash Point |
214.3±12.4 °C
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| Vapour Pressure |
0.0±2.8 mmHg at 25°C
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| Index of Refraction |
1.522
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| LogP |
10.2
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
29
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| Complexity |
620
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| Defined Atom Stereocenter Count |
9
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| SMILES |
O([H])[C@@]1([H])C([H])([H])C([H])([H])[C@@]2(C([H])([H])[H])C(C1([H])[H])=C([H])C([H])([H])[C@]1([H])[C@@]2([H])C([H])([H])C([H])([H])[C@]2(C([H])([H])[H])[C@@]([H])([C@]([H])(C([H])([H])[H])C([H])([H])C([H])([H])[C@]([H])(C([H])([H])[H])C([H])(C([H])([H])[H])C([H])([H])[H])C([H])([H])C([H])([H])[C@]21[H]
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| InChi Key |
SGNBVLSWZMBQTH-PODYLUTMSA-N
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| InChi Code |
InChI=1S/C28H48O/c1-18(2)19(3)7-8-20(4)24-11-12-25-23-10-9-21-17-22(29)13-15-27(21,5)26(23)14-16-28(24,25)6/h9,18-20,22-26,29H,7-8,10-17H2,1-6H3/t19-,20-,22+,23+,24-,25+,26+,27+,28-/m1/s1
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| Chemical Name |
(3S,8S,9S,10R,13R,14S,17R)-17-[(2R,5R)-5,6-dimethylheptan-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol
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| Synonyms |
(24R-5-Ergosten-3β-olCampesterol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol : ~9.09 mg/mL (~22.69 mM)
H2O : ~1 mg/mL (~2.50 mM) DMSO : ~0.67 mg/mL (~1.67 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 0.91 mg/mL (2.27 mM) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.1 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.91 mg/mL (2.27 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.1 mg/mL clear EtOH stock solution to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4958 mL | 12.4788 mL | 24.9576 mL | |
| 5 mM | 0.4992 mL | 2.4958 mL | 4.9915 mL | |
| 10 mM | 0.2496 mL | 1.2479 mL | 2.4958 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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