| Size | Price | Stock | Qty |
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| 2mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
Calf thymus DNA does not have a specific pharmacological target, as it is a research reagent. Its primary function is to serve as a model substrate for studying DNA interactions. In vitro, it is used to investigate the binding of various compounds to DNA, including intercalators, groove binders, and alkylating agents. It is also used as a standard in DNA quantification assays and as a template for PCR.
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| ln Vitro |
Techniques such as emission, absorption, circular dichroism (CD), and viscosity can be used to examine the relationship between calf thymus DNA and the antiviral medication lamivudine [2].
In vitro, Calf thymus DNA is characterized by its physical and chemical properties. Its UV absorbance at 260 nm is used to quantify DNA concentration. Its melting temperature (Tm) and hyperchromicity are used to assess its integrity and double-stranded nature. It is used as a substrate in assays to measure the activity of enzymes that act on DNA, such as nucleases, polymerases, and topoisomerases. |
| ln Vivo |
In vivo, Calf thymus DNA is not used as a therapeutic agent. Its application is strictly in vitro and ex vivo research. However, DNA itself can be used as a therapeutic agent in gene therapy, where it is delivered to cells to correct genetic defects or to express therapeutic proteins.
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| Enzyme Assay |
Calf thymus DNA is a highly purified, double-stranded DNA. Its purity and integrity are assessed by measuring the A260/A280 ratio (typically 1.8-2.0) and by agarose gel electrophoresis. It is typically provided as a lyophilized powder or as a solution in TE buffer. It is stored at -20°C to maintain its stability and prevent degradation.
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| Cell Assay |
Calf thymus DNA is used as a substrate in cell-free assays to study DNA-protein interactions. It is also used as a carrier DNA in transfection experiments to increase the efficiency of gene delivery. In cell culture, it can be used to study the cellular uptake of DNA and the activation of DNA sensors, such as cGAS-STING pathway.
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| Animal Protocol |
In vivo animal experiments with Calf thymus DNA are not common. However, DNA from various sources can be used in animal models of autoimmune diseases, such as systemic lupus erythematosus (SLE), to study the immune response to DNA. Calf thymus DNA may be used as a control or standard in such studies.
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| ADME/Pharmacokinetics |
Calf thymus DNA is a high molecular weight polymer with a molecular weight typically ranging from 5-15 million Daltons. It is soluble in water and TE buffer. It is typically stored as a lyophilized powder or in solution at -20°C. Its stability is maintained under recommended storage conditions. It is a hygroscopic compound and should be protected from moisture.
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| Toxicity/Toxicokinetics |
Calf thymus DNA is considered to be non-toxic, as it is a natural biological molecule. However, as a research reagent, it is not intended for human therapeutic use. Standard safety precautions for handling biochemical reagents should be followed.
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| References | |
| Additional Infomation |
Calf thymus DNA is a research compound with no clinical approval. It is a widely used standard and substrate in molecular biology and biochemistry. It is used in DNA quantification, as a template for PCR, in DNA-protein interaction studies, and in the development of DNA-based biosensors. It is also used as a carrier DNA in transfection experiments.
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| Molecular Formula |
C11H9N3O2.NA+
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|---|---|
| Molecular Weight |
238.19786
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| CAS # |
91080-16-9
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| Appearance |
White to light yellow solid powder
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| SMILES |
[Calf thymus DNA]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL
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| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1982 mL | 20.9908 mL | 41.9815 mL | |
| 5 mM | 0.8396 mL | 4.1982 mL | 8.3963 mL | |
| 10 mM | 0.4198 mL | 2.0991 mL | 4.1982 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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