| Size | Price | Stock | Qty |
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| ln Vitro |
Calenduloside E exhibited anti-apoptotic activity in H2O2-induced PC12 cells, inhibiting apoptosis in a dose-dependent manner (10, 20, 40 μM) [1]
It significantly reduced the apoptotic rate of PC12 cells induced by H2O2, as detected by Annexin V-FITC/PI double staining [1] The compound upregulated the expression of anti-apoptotic protein Bcl-2 and downregulated the expression of pro-apoptotic proteins Bax and cleaved caspase-3 in H2O2-treated PC12 cells, as confirmed by Western blot analysis [1] The clickable activity-based probe derived from Calenduloside E specifically bound to its intracellular targets in PC12 cells, enabling target capture and identification via click chemistry and mass spectrometry [1] |
|---|---|
| Cell Assay |
PC12 cells were cultured in DMEM medium supplemented with fetal bovine serum and antibiotics, maintained at 37°C in a 5% CO2 incubator [1]
Anti-apoptotic activity assay: PC12 cells were seeded in 6-well plates, allowed to adhere overnight, pretreated with Calenduloside E (10, 20, 40 μM) for 2 hours, then stimulated with H2O2 (200 μM) to induce apoptosis for 24 hours [1] - Flow cytometry analysis: Cells were harvested, washed with phosphate-buffered saline, stained with Annexin V-FITC and PI for 15 minutes in the dark, and apoptotic rate was detected by flow cytometer [1] - Western blot analysis: Cells were lysed in RIPA buffer containing protease inhibitors, protein concentrations were determined by BCA assay, equal amounts of protein were separated by SDS-PAGE, transferred to PVDF membranes, incubated with primary antibodies against Bcl-2, Bax, cleaved caspase-3, and β-actin (loading control) overnight at 4°C, followed by HRP-conjugated secondary antibodies, and protein bands were visualized using an enhanced chemiluminescence system [1] Clickable probe-based target capture assay: PC12 cells were incubated with the clickable activity-based probe of Calenduloside E for 4 hours at 37°C [1] - Cells were harvested, lysed, and the cell lysate was subjected to click reaction with a fluorescent tag under specific conditions [1] - The probe-target protein complexes were captured by immunoprecipitation, separated by SDS-PAGE, and the target proteins were identified by mass spectrometry [1] |
| References | |
| Additional Infomation |
Oleanolic acid 3-O-β-D-glucuronic acid is a β-D-glucuronic acid whose function is related to oleanolic acid.
It has been reported that calendula glycoside E is found in quinoa, calendula, and other organisms with relevant data. See also: Calendula (partial). Calendula glycoside E is a natural bioactive compound with anti-apoptotic properties[1]. Clickable active probes designed based on calendula glycoside E can be used to identify and characterize its intracellular molecular targets[1]. Its anti-apoptotic mechanism is related to the regulation of Bcl-2 family proteins and the caspase-dependent apoptosis signaling pathway[1]. |
| Molecular Formula |
C36H56O9
|
|---|---|
| Molecular Weight |
632.8245
|
| Exact Mass |
632.392
|
| CAS # |
26020-14-4
|
| PubChem CID |
176079
|
| Appearance |
White to off-white solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
750.8±60.0 °C at 760 mmHg
|
| Melting Point |
243-245 ºC (decomp)
|
| Flash Point |
228.6±26.4 °C
|
| Vapour Pressure |
0.0±5.7 mmHg at 25°C
|
| Index of Refraction |
1.590
|
| LogP |
7.04
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
45
|
| Complexity |
1260
|
| Defined Atom Stereocenter Count |
13
|
| SMILES |
C[C@]12CC[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CC=C4[C@]3(CC[C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)C)C)(C)C)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)C(=O)O)O)O)O
|
| InChi Key |
IUCHKMAZAWJNBJ-RCYXVVTDSA-N
|
| InChi Code |
InChI=1S/C36H56O9/c1-31(2)14-16-36(30(42)43)17-15-34(6)19(20(36)18-31)8-9-22-33(5)12-11-23(32(3,4)21(33)10-13-35(22,34)7)44-29-26(39)24(37)25(38)27(45-29)28(40)41/h8,20-27,29,37-39H,9-18H2,1-7H3,(H,40,41)(H,42,43)/t20-,21-,22+,23-,24-,25-,26+,27-,29+,33-,34+,35+,36-/m0/s1
|
| Chemical Name |
(2S,3S,4S,5R,6R)-6-[[(3S,4aR,6aR,6bS,8aS,12aS,14aR,14bR)-8a-carboxy-4,4,6a,6b,11,11,14b-heptamethyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicen-3-yl]oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~158.02 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5802 mL | 7.9011 mL | 15.8023 mL | |
| 5 mM | 0.3160 mL | 1.5802 mL | 3.1605 mL | |
| 10 mM | 0.1580 mL | 0.7901 mL | 1.5802 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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