| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
P53 and C16-ceramide interact within the core domain of the latter. In cells, p53 combines with natural C16-ceramide to form a complex [1]. HCT116 cell viability is significantly decreased by C16-ceramide (2.5–50 μM; 0-48 hours) in a concentration- and time-dependent manner [2]. Btf (Bcl-2-related transcription factor) is the mechanism by which C16-ceramide (12 μM; 48 hours) causes apoptosis in HCT116 cells [2]. p53 and BAX expression are upregulated by C16-ceramide (12 μM; 0–6 hours) and Btf expression. Through Btf, C16-ceramide inhibits the expression of Mdm2 [2].
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| Cell Assay |
Cell Viability Assay[2]
Cell Types: HCT116 Cell Tested Concentrations: 2.5, 5, 10, 12, 20, 50 µM Incubation Duration: 0-48 hrs (hours) Experimental Results: Cell viability diminished dramatically in a time and concentration dependent manner. Western Blot Analysis[2] Cell Types: HCT116 Cell Tested Concentrations: 12 μM Incubation Duration: 1, 3 and 6 hrs (hours) Experimental Results: Increased PARP cleavage and diminished pro-caspase 3. diminished stratifin and stathmin levels, increased inhibin and btw. After treatment, RNAi-mediated Btf depletion also partially suppressed BAX expression. Luciferase activity and Mdm2 protein expression levels were Dramatically diminished. |
| References | |
| Additional Infomation |
N-hexadecanoylsphingosine is a N-acylsphingosine in which the ceramide N-acyl group is specified as hexadecanoyl (palmitoyl). It has a role as a Mycoplasma genitalium metabolite and a human blood serum metabolite. It is a N-acylsphingosine, a Cer(d34:1) and a N-palmitoyl-sphingoid base. It is functionally related to a hexadecanoic acid.
N-Palmitoylsphingosine has been reported in Junceella juncea and Trypanosoma brucei with data available. |
| Molecular Formula |
C34H67NO3
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|---|---|
| Molecular Weight |
537.900691270828
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| Exact Mass |
537.512
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| CAS # |
24696-26-2
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| Related CAS # |
C16-Ceramide-d31;852043-41-5;C16-Ceramide-13C16;C16-Ceramide-d9;2260669-51-8
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| PubChem CID |
5283564
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| Appearance |
White to off-white solid powder
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| Density |
0.919g/cm3
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| Boiling Point |
675.396ºC at 760 mmHg
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| Melting Point |
94-95ºC
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| Flash Point |
362.267ºC
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| Vapour Pressure |
0mmHg at 25°C
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| Index of Refraction |
1.48
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| LogP |
9.953
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
30
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| Heavy Atom Count |
38
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| Complexity |
508
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| Defined Atom Stereocenter Count |
2
|
| SMILES |
CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@@H](/C=C/CCCCCCCCCCCCC)O
|
| InChi Key |
YDNKGFDKKRUKPY-TURZORIXSA-N
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| InChi Code |
InChI=1S/C34H67NO3/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-33(37)32(31-36)35-34(38)30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h27,29,32-33,36-37H,3-26,28,30-31H2,1-2H3,(H,35,38)/b29-27+/t32-,33+/m0/s1
|
| Chemical Name |
N-[(E,2S,3R)-1,3-dihydroxyoctadec-4-en-2-yl]hexadecanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol : ~20 mg/mL (~37.18 mM; warming and heat to 60°C) DMF : 20 mg/mL (~37.18 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2 mg/mL (3.72 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear EtOH + stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8591 mL | 9.2954 mL | 18.5908 mL | |
| 5 mM | 0.3718 mL | 1.8591 mL | 3.7182 mL | |
| 10 mM | 0.1859 mL | 0.9295 mL | 1.8591 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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