| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg | |||
| 500mg | |||
| Other Sizes |
| Targets |
Bromobimane targets thiol (sulfhydryl) groups in proteins, peptides, and other biomolecules. As a fluorescent labeling reagent, it reacts specifically with thiol groups to form highly fluorescent adducts. This reaction is widely used for the detection and quantification of thiol-containing compounds in various biological samples. The compound does not have a specific therapeutic target but is a valuable tool for studying thiol metabolism and protein structure.
|
|---|---|
| ln Vitro |
Bromobimane (mBBr) marks reactive thiols in cells [1] (1) Use ethylbenzaldehyde 100 mM mBBr solution. (2) Add 1 mL of 10% cell suspension to 15-25 μL of 100 mM mBBr solution. (3) After 30-45 minutes at 37°C, perform fluorescence detection.
In vitro, Bromobimane is used as a fluorescent labeling reagent for the detection and quantification of thiols. It reacts specifically with thiol groups to form highly fluorescent adducts, which can be detected by fluorescence spectroscopy or HPLC with fluorescence detection. The compound is used in studies of thiol metabolism, protein structure, and redox biology. Its fluorescence properties make it a sensitive probe for thiol-containing compounds. |
| ln Vivo |
In vivo, Bromobimane is not used as a therapeutic agent. Its application is limited to in vitro research applications. The compound is not administered to animals or humans for therapeutic purposes. Its use is strictly confined to laboratory settings for thiol detection.
|
| Enzyme Assay |
In vitro non-cell assays for Bromobimane involve its use as a fluorescent labeling reagent for thiols. The compound is incubated with a sample containing thiol-containing compounds, and the fluorescence of the resulting adducts is measured. This is used to quantify thiols in biological samples, such as protein solutions or cell lysates. The reaction is specific and sensitive, making it a valuable tool for thiol analysis.
|
| Cell Assay |
In vitro cell-based assays for Bromobimane involve labeling thiol-containing proteins in cells. Cells are incubated with the compound, and the fluorescence of the labeled proteins is detected by fluorescence microscopy or flow cytometry. This is used to study thiol metabolism and redox status in cells. The compound's cell permeability allows for intracellular labeling of thiols.
|
| Animal Protocol |
In vivo animal studies are not typically performed with Bromobimane, as it is a fluorescent labeling reagent and not a therapeutic agent. Its use is limited to in vitro applications.
|
| ADME/Pharmacokinetics |
Metabolism / Metabolites
Bromine is primarily absorbed through inhalation, but can also enter the body through skin contact. Bromine salts can be ingested. Due to its reactivity, bromine rapidly forms bromides and may deposit in tissues, displacing other halogens. (L626) Bromobimane has a molecular weight of 271.11 g/mol and a molecular formula of C₁₀H₁₁BrN₂O₂. It appears as a light yellow to yellow to orange powder to crystal. It has a melting point of 159.0 to 163.0 °C. It is light and heat sensitive and should be stored at <0°C. The compound is soluble in DMSO and other organic solvents. |
| Toxicity/Toxicokinetics |
Toxicity Summary
Bromine is a strong oxidizing agent that can release oxygen free radicals from water in mucous membranes. These free radicals are also strong oxidizing agents and can cause tissue damage. Furthermore, bromine can generate hydrobromic acid and bromic acid, leading to secondary irritation. Bromine ions can also affect the central nervous system, causing bromine poisoning. This is believed to be due to bromide ions replacing chloride ions in the action of neurotransmitters and transport systems, thereby affecting various synaptic processes. (L626, L627, A543) Bromobimane is considered a safe laboratory reagent when handled appropriately. It is not intended for human or veterinary use. Standard laboratory safety precautions should be followed when handling the compound, including the use of appropriate personal protective equipment. |
| References | |
| Additional Infomation |
Monobromodiamine is a pyrazolopyrazole compound composed of 1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione, with three methyl groups at positions 2, 5, and 6, and a bromomethyl group at position 3. It can be used as a fluorescent dye. It is an organobromine compound and also a pyrazolopyrazole compound. Bromodiamine is an organobromine compound. Bromine is a halogen element with the symbol Br and atomic number 35. Diatomic bromine does not exist in nature, but bromine salts exist in Earth's crustal rocks. (L625)
Bromobimane is a fluorescent labeling reagent used for the detection and quantification of thiols. It is suitable for fluorescence applications and is a BioReagent. Not a therapeutic agent; intended for research purposes only. |
| Molecular Formula |
C10H11BRN2O2
|
|---|---|
| Molecular Weight |
271.1105
|
| Exact Mass |
270
|
| CAS # |
71418-44-5
|
| PubChem CID |
114810
|
| Appearance |
Light yellow to yellow solid powder
|
| Density |
1.66g/cm3
|
| Boiling Point |
327.8ºC at 760 mmHg
|
| Melting Point |
152-154ºC
|
| Flash Point |
152.1ºC
|
| Index of Refraction |
1.64
|
| LogP |
1.017
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
15
|
| Complexity |
437
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
AHEWZZJEDQVLOP-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
|
| Chemical Name |
7-(bromomethyl)-1,2,6-trimethylpyrazolo[1,2-a]pyrazole-3,5-dione
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 86.6 mg/mL (~319.43 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6885 mL | 18.4427 mL | 36.8854 mL | |
| 5 mM | 0.7377 mL | 3.6885 mL | 7.3771 mL | |
| 10 mM | 0.3689 mL | 1.8443 mL | 3.6885 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
