| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
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| Other Sizes |
| ln Vitro |
Bromobimane (mBBr) marks reactive thiols in cells [1] (1) Use ethylbenzaldehyde 100 mM mBBr solution. (2) Add 1 mL of 10% cell suspension to 15-25 μL of 100 mM mBBr solution. (3) After 30-45 minutes at 37°C, perform fluorescence detection.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
Bromine is primarily absorbed through inhalation, but can also enter the body through skin contact. Bromine salts can be ingested. Due to its reactivity, bromine rapidly forms bromides and may deposit in tissues, displacing other halogens. (L626) |
| Toxicity/Toxicokinetics |
Toxicity Summary
Bromine is a strong oxidizing agent that can release oxygen free radicals from water in mucous membranes. These free radicals are also strong oxidizing agents and can cause tissue damage. Furthermore, bromine can generate hydrobromic acid and bromic acid, leading to secondary irritation. Bromine ions can also affect the central nervous system, causing bromine poisoning. This is believed to be due to bromide ions replacing chloride ions in the action of neurotransmitters and transport systems, thereby affecting various synaptic processes. (L626, L627, A543) |
| References | |
| Additional Infomation |
Monobromodiamine is a pyrazolopyrazole compound composed of 1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione, with three methyl groups at positions 2, 5, and 6, and a bromomethyl group at position 3. It can be used as a fluorescent dye. It is an organobromine compound and also a pyrazolopyrazole compound. Bromodiamine is an organobromine compound. Bromine is a halogen element with the symbol Br and atomic number 35. Diatomic bromine does not exist in nature, but bromine salts exist in Earth's crustal rocks. (L625)
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| Molecular Formula |
C10H11BRN2O2
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|---|---|
| Molecular Weight |
271.1105
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| Exact Mass |
270
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| CAS # |
71418-44-5
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| PubChem CID |
114810
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.66g/cm3
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| Boiling Point |
327.8ºC at 760 mmHg
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| Melting Point |
152-154ºC
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| Flash Point |
152.1ºC
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| Index of Refraction |
1.64
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| LogP |
1.017
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
15
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| Complexity |
437
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
AHEWZZJEDQVLOP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
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| Chemical Name |
7-(bromomethyl)-1,2,6-trimethylpyrazolo[1,2-a]pyrazole-3,5-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 86.6 mg/mL (~319.43 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6885 mL | 18.4427 mL | 36.8854 mL | |
| 5 mM | 0.7377 mL | 3.6885 mL | 7.3771 mL | |
| 10 mM | 0.3689 mL | 1.8443 mL | 3.6885 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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