| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
|
||
| 25g |
|
||
| 50g |
|
||
| 100g |
|
||
| Other Sizes |
Purity: ≥98%
| Targets |
Bovine Serum Albumin does not have a specific pharmacological target. It functions as a carrier protein, a blocking agent, and a stabilizer. It binds to various molecules, including fatty acids, hormones, and drugs, and is used to stabilize proteins and enzymes in solution. It is also used as a blocking agent to prevent non-specific binding in immunoassays.
|
|---|---|
| ln Vitro |
In vitro, Bovine Serum Albumin is used for a wide range of applications. It is used as a standard in protein quantification assays, such as the Bradford assay. It is used as a blocking agent in immunohistochemistry and ELISA to prevent non-specific binding. It is also used to stabilize enzymes and other proteins in solution and to supplement cell culture media.
|
| ln Vivo |
In vivo, Bovine Serum Albumin is not used as a therapeutic agent. It is used as a component of some diagnostic reagents and as a stabilizer in pharmaceutical formulations. It is also used in research to study protein-protein interactions and as a model protein for studying protein folding and stability.
|
| Enzyme Assay |
The in vitro assay for Bovine Serum Albumin is not a biological assay but an analytical one. Its concentration is measured using the Bradford assay, which relies on the binding of Coomassie Brilliant Blue dye to the protein. Its purity is assessed by SDS-PAGE. Its ability to bind to various molecules can be studied using equilibrium dialysis or isothermal titration calorimetry (ITC).
|
| Cell Assay |
In vitro cell culture studies for Bovine Serum Albumin utilize it as a supplement in cell culture media. It provides a source of protein and helps to stabilize other components in the media. It is also used to study the uptake and transport of fatty acids and other molecules by cells.
|
| Animal Protocol |
In vivo animal experiments for Bovine Serum Albumin are not common. However, it is sometimes used as a carrier protein for the administration of hydrophobic compounds in animal studies. It can also be used as a model antigen to study the immune response.
|
| ADME/Pharmacokinetics |
Bovine Serum Albumin is a protein with a molecular weight of approximately 66.4 kDa and a molecular formula of C2934H4581N781O897S39. It is a lyophilized powder that is soluble in water and aqueous buffers. It is typically stored at 4°C or -20°C for long-term stability. Its stability is maintained under recommended storage conditions.
|
| Toxicity/Toxicokinetics |
Toxicological data for Bovine Serum Albumin indicate that it is generally non-toxic. It is a naturally occurring protein that is widely used in research and as a food additive. It is not intended for human therapeutic use as a drug, although it is used as a component of some pharmaceutical formulations.
|
| References | |
| Additional Infomation |
Bovine Serum Albumin is a research reagent with no clinical approval as a drug. It is one of the most widely used proteins in biochemistry and molecular biology. It is used as a standard in protein assays, as a blocking agent in immunoassays, as a stabilizer for enzymes, and as a supplement in cell culture media.
|
| Molecular Formula |
C3F8
|
|---|---|
| Molecular Weight |
188.019329071045
|
| CAS # |
9048-46-8
|
| Appearance |
White to light yellow solid powder
|
| SMILES |
FC(C(F)(F)F)(C(F)(F)F)F
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~50 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3186 mL | 26.5929 mL | 53.1858 mL | |
| 5 mM | 1.0637 mL | 5.3186 mL | 10.6372 mL | |
| 10 mM | 0.5319 mL | 2.6593 mL | 5.3186 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA