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| 5mg |
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| Other Sizes |
| Targets |
The compound is an amine-reactive fluorescent probe. Its "target" is any primary amine (-NH2) group on a biomolecule. Once conjugated, the BODIPY fluorophore serves as a detection agent, not a modulator of protein function. The BODIPY core is known for its unique hydrophobic properties, making it ideal for labeling lipids, membranes, and other lipophilic compounds, as well as general protein labeling.
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| ln Vitro |
As a fluorescent dye, this compound does not have inhibitory or agonist activity. Its primary property is its bright fluorescence and stability. It is used to create fluorescent conjugates, which are then used to track, quantify, or localize specific target molecules in vitro. The dye is characterized by its high quantum yield, photostability, and insensitivity to solvent polarity and pH.
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| ln Vivo |
As a labeling reagent, BODIPY-581/591 NHS ester is not used for in vivo activity studies. Its conjugates can be used in vivo for intravital imaging or flow cytometry, but the dye itself is a tool for generating those tracers. For example, a BODIPY-labeled antibody can be injected into a mouse to visualize tumor-specific antigens via fluorescence imaging. An in vivo protocol would involve preparing a sterile solution of the labeled conjugate and performing intravenous injection.
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| Enzyme Assay |
This is a chemical labeling reaction, not a biomolecular binding assay. The efficiency of the NHS ester is tested by reacting the dye with a model amine (e.g., butylamine) in an appropriate buffer (e.g., 0.1 M NaHCO3, pH 8.5) at room temperature for 1 hour. The reaction progress is monitored by thin-layer chromatography (TLC) or HPLC. The dye's fluorescence is not altered by the reaction; the labeling efficiency is determined by the increase in molecular weight.
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| Cell Assay |
The labeled conjugate is used in cellular assays. For example, a BODIPY-labeled antibody targeting a cell surface marker (e.g., CD8) is incubated with live cells on ice for 30 minutes. After washing away unbound antibody, the cells are analyzed by flow cytometry to quantify the percentage of CD8+ cells. For imaging, cells are incubated with BODIPY-labeled secondary antibody or labeled toxin, fixed, and mounted on slides for confocal microscopy (Ex/Em: 581/591 nm).
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| Animal Protocol |
The labeled conjugate itself is used for in vivo imaging. A BODIPY-conjugated antibody (e.g., anti-HER2) is prepared and purified. The conjugate is then injected intravenously (e.g., 100 ug per mouse) into a mouse bearing a HER2-positive xenograft tumor. Mice are imaged at various time points (e.g., 24, 48, 72 hours post-injection) using an in vivo fluorescence imaging system equipped with a 581 nm excitation source and a 591 nm emission filter to assess the biodistribution and tumor targeting of the antibody.
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| ADME/Pharmacokinetics |
BODIPY-581/591 NHS ester is a small molecule dye (MW 489.28). When conjugated to an antibody, the PK of the conjugate is governed by the protein, not the dye. The dye itself is extremely hydrophobic and, if not conjugated, would aggregate. Therefore, its free form is not administered in vivo. The key property is the stability of the amide bond formed between the dye and the amine.
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| Toxicity/Toxicokinetics |
BODIPY dyes are generally considered to have low acute toxicity. The NHS ester group is reactive and should be handled carefully as it can cause irritation. When conjugated to antibodies or other targeting moieties, the toxicity is dictated by the conjugated molecule. Standard laboratory safety practices for handling reactive chemicals and for disposing of fluorescent dyes should be followed.
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| References | |
| Additional Infomation |
BODIPY-581/591 NHS ester is a research-grade chemical widely used in molecular biology and cell biology. It is not a drug or a drug candidate. It is a tool for creating fluorescent reporters. The NHS ester chemistry is one of the most common and robust methods for protein labeling, and this dye's spectral properties make it well-suited for multicolor imaging experiments, especially as a red channel in flow cytometry and immunofluorescence.
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| Molecular Formula |
C26H22BN3O4F2
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|---|---|
| Molecular Weight |
489.278
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| CAS # |
150152-70-8
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| Appearance |
Pale purple to purple solid powder
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| SMILES |
[F-][B+3]1(N2=C(C=CC2=CC2=CC=C(CCC(=O)ON3C(CCC3=O)=O)[N-]12)C=CC=CC1C=CC=CC=1)[F-]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~51.10 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 1.25 mg/mL (2.55 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0438 mL | 10.2191 mL | 20.4382 mL | |
| 5 mM | 0.4088 mL | 2.0438 mL | 4.0876 mL | |
| 10 mM | 0.2044 mL | 1.0219 mL | 2.0438 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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