| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
BMS-688521 in a mouse-specific adhesion assay with mouse splenocytes and the cell line b.END3 that expresses ICAM-1. The human T cell/HUVEC detection data (ICIC50=2.5 nM) is approximately 30 times higher than the IC50 of BMS-688521, which is 78 nM [1].
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|---|---|
| ln Vivo |
In a mouse model of allergic eosinophilic lung inflammation, BMS-688521 (1–10 mg/kg; oral; three days BID) can effectively reduce lung inflammation [1]. Cmax, Tmax, AUC, and F values of BMS-688521 (5 mg/kg; oral) were 0.32 μM, 1.0 μM, 1.5 μM h, and 50%, correspondingly [1]. BALB/c mice treated with 1 mg/kg of BMS-688521 (iv) had T1/2, MRT, CL, and Vss values of 1.6 hours, 1.7 hours, 50 mL/mim/kg, and 5.1 L/kg, respectively [1].
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| Animal Protocol |
Animal/Disease Models: BALB/c female mice, 6-8 weeks old (OVA mouse lung inflammation) [1]
Doses: 1, 3 and 10 mg/kg Doses: Oral; three days BID Experimental Results: 1 mg BID /kg dose Dramatically inhibited eosinophil accumulation in a dose-dependent manner at 3 mg/kg and 10 mg/kg. |
| References |
| Molecular Formula |
C26H19CL2N5O4
|
|---|---|
| Molecular Weight |
536.37
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| Exact Mass |
535.081
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| CAS # |
893397-44-9
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| PubChem CID |
11699447
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| Appearance |
White to light yellow solid powder
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| LogP |
4.467
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
37
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| Complexity |
971
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CN1C(=O)N(C(=O)[C@]12CN(C[C@H]2C3=CC=C(C=C3)C#N)C4=NC=C(C=C4)C(=O)O)C5=CC(=CC(=C5)Cl)Cl
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| InChi Key |
LILGMDXLRPEBNH-HFZDXXHNSA-N
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| InChi Code |
InChI=1S/C26H19Cl2N5O4/c1-31-25(37)33(20-9-18(27)8-19(28)10-20)24(36)26(31)14-32(22-7-6-17(12-30-22)23(34)35)13-21(26)16-4-2-15(11-29)3-5-16/h2-10,12,21H,13-14H2,1H3,(H,34,35)/t21-,26+/m0/s1
|
| Chemical Name |
6-[(5S,9R)-9-(4-cyanophenyl)-3-(3,5-dichlorophenyl)-1-methyl-2,4-dioxo-1,3,7-triazaspiro[4.4]nonan-7-yl]pyridine-3-carboxylic acid
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| Synonyms |
BMS688521; BMS 688521; BMS-688521
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~186.44 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (9.32 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (9.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8644 mL | 9.3219 mL | 18.6438 mL | |
| 5 mM | 0.3729 mL | 1.8644 mL | 3.7288 mL | |
| 10 mM | 0.1864 mL | 0.9322 mL | 1.8644 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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