| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
BMPS does not have a specific biological target. It is a chemical crosslinking reagent used to covalently link molecules containing primary amines (e.g., lysine residues in proteins) and sulfhydryl groups (e.g., cysteine residues). Its "targets" are these functional groups on biomolecules. By reacting with both an amine and a thiol, BMPS forms a stable amide bond and a stable thioether bond, creating a covalent linkage between two molecules.
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|---|---|
| ln Vitro |
The in vitro activity of BMPS is defined by its chemical reactivity and crosslinking efficiency. Its ability to form stable conjugates between amine-containing and thiol-containing molecules is assessed in various bioconjugation assays. The compound's utility as a crosslinker is evaluated by measuring the yield and purity of the resulting conjugates using techniques such as SDS-PAGE, HPLC, or mass spectrometry. The stability of the conjugates under various conditions is also assessed.
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| ln Vivo |
BMPS is not a therapeutic agent and is not administered in vivo. It is used as a research tool for the synthesis of bioconjugates, including antibody-drug conjugates (ADCs). The compounds synthesized using BMPS may be evaluated in vivo, but the crosslinker itself is not. Its role is to provide a stable, non-cleavable linkage between the antibody and the payload in ADC development.
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| Enzyme Assay |
The reactivity of BMPS is assessed using chemical conjugation assays. The NHS ester is reacted with a primary amine-containing compound (e.g., a model amine or a protein), and the maleimide is reacted with a thiol-containing compound (e.g., a model thiol or a peptide). The formation of the conjugates is monitored by analytical techniques such as HPLC, mass spectrometry, or SDS-PAGE. The efficiency of crosslinking is determined by measuring the degree of conjugation.
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| Cell Assay |
BMPS is used in the synthesis of bioconjugates for cellular assays. For example, it can be used to conjugate a targeting ligand to a fluorescent dye or a cytotoxic payload. The resulting conjugate can be tested for cellular uptake, binding affinity, and biological activity in cell-based assays. The crosslinker's non-cleavable nature ensures that the conjugate remains intact within the cell.
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| Animal Protocol |
BMPS is not administered in animal models. It is used to synthesize conjugates that are subsequently tested in vivo. The linker's stability and its effect on the pharmacokinetics and pharmacodynamics of the conjugate are evaluated through the performance of the conjugate in animal studies. The non-cleavable nature of BMPS may provide advantages in terms of conjugate stability in circulation.
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| ADME/Pharmacokinetics |
BMPS has a molecular weight of 266.21 g/mol and a molecular formula of C11H10N2O6. It is a white to off-white solid powder with a melting point of 168-170°C. It is soluble in DMSO at ≥45 mg/mL. The compound should be stored as a powder at -20°C for up to 3 years or in solvent at -80°C for up to 6 months, in a sealed and protected environment (e.g., under nitrogen) to avoid exposure to moisture.
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| Toxicity/Toxicokinetics |
BMPS is a chemical reagent and is not intended for human use. It can cause skin and eye irritation upon contact. The compound should be handled with appropriate safety precautions, including the use of gloves, safety glasses, and proper ventilation. It is not a pharmaceutical drug and is not intended for therapeutic use. Standard laboratory safety precautions should be followed when handling the compound.
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| Additional Infomation |
BMPS (N-Succinimidyl 3-maleimidopropionate) is a non-cleavable, heterobifunctional crosslinker commonly used in ADC linker design. It features an NHS ester reactive toward primary amines and a maleimide reactive toward sulfhydryl groups. BMPS is used to prepare antibody-conjugated active molecules (ADCs). The compound is not approved for clinical use and is intended for research purposes only.
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| Molecular Formula |
C11H10N2O6
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|---|---|
| Molecular Weight |
266.21
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| Exact Mass |
266.054
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| CAS # |
55750-62-4
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| PubChem CID |
4620597
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| Appearance |
White to off-white solid powder
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| Density |
1.55g/cm3
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| Boiling Point |
448.8ºC at 760mmHg
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| Melting Point |
168-170 °C(lit.)
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| Flash Point |
225.2ºC
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| Index of Refraction |
1.605
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| LogP |
-1.6
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| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
19
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| Complexity |
475
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
JKHVDAUOODACDU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H10N2O6/c14-7-1-2-8(15)12(7)6-5-11(18)19-13-9(16)3-4-10(13)17/h1-2H,3-6H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-(2,5-dioxopyrrol-1-yl)propanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 45 mg/mL (~169.04 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7564 mL | 18.7822 mL | 37.5643 mL | |
| 5 mM | 0.7513 mL | 3.7564 mL | 7.5129 mL | |
| 10 mM | 0.3756 mL | 1.8782 mL | 3.7564 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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