| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 500mg |
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| Other Sizes |
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| ln Vitro |
By capturing •OH and GS•, BMPO can create more long-lasting BMPO/•OH and BMPO/•SG adducts. Additionally, in biological systems, the electron spin resonance (ESR) spectra of adducts produced from BMPO show a good signal-to-noise ratio. For instance, the hydroxyl adduct of BMPO glutathione adduct (BMPO/·SG) and its ESR spectrum do not entirely coincide. BMPO is perfect for the detection of superoxide anions, hydroxyl, and sulfide radicals due to its good chemical and spectroscopic qualities. [1] Two cell viability estimations were used to quantify the possible harmful effects of spin trap BMPO (trypan blue exclusion and colony formation). [3] In CHO cells, BMPO at 2.5 or 25 mM had no discernible impact on cell viability. Nonetheless, it led to a notable rise in the quantity of cells that were incapable of excluding 50 mM BMPO trypan blue. At a 25 mM dose, BMPO dramatically decreased colony formation in CHO cells by 30%. Additionally, at 50 mM, BMPO entirely blocked the formation of colonies in 9L tumor cells. [3] also evaluated the rate at which CHO cells consumed oxygen, and BMPO (25 mM) considerably decreased this rate. [3]
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| References |
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| Molecular Formula |
C10H17NO3
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|---|---|
| Molecular Weight |
199.246883153915
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| Exact Mass |
199.12
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| CAS # |
387334-31-8
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| PubChem CID |
11241066
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
0.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
14
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| Complexity |
278
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C(C)(C)C)C(C1(C)CCC=[N+]1[O-])=O
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| InChi Key |
MDMUYJRRYYXDLZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H17NO3/c1-9(2,3)14-8(12)10(4)6-5-7-11(10)13/h7H,5-6H2,1-4H3
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| Chemical Name |
tert-butyl 2-methyl-1-oxido-3,4-dihydropyrrol-1-ium-2-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~501.88 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.55 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (12.55 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0188 mL | 25.0941 mL | 50.1882 mL | |
| 5 mM | 1.0038 mL | 5.0188 mL | 10.0376 mL | |
| 10 mM | 0.5019 mL | 2.5094 mL | 5.0188 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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