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| Other Sizes |
| Targets |
Bis-tris propane does not have a specific biological target. It is a zwitterionic buffer used to maintain pH in biochemical and biological experiments. It is a water-soluble buffer substance used for the preparation of biochemical and biological buffer solutions. Bis-tris propane is unique among biological buffers because it can buffer across a wide pH range.
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| ln Vitro |
Bis-tris propane does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and biological research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications.
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| ln Vivo |
Bis-tris propane does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications.
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| Enzyme Assay |
In vitro biochemical assays using Bis-tris propane buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH within its effective range (6.3-9.5). Bis-tris propane is used in various biochemical and biological applications where a buffer with a wide pH range is needed.
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| Cell Assay |
Cell culture experiments using Bis-tris propane are possible as its pH range (6.3-9.5) covers physiological pH. It can be used in cell culture media at appropriate concentrations. The compound's unique buffering properties may be advantageous in specific cell culture applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to Bis-tris propane as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Bis-tris propane as it is a buffer reagent rather than a therapeutic agent. The compound has a molecular weight of 282.33, a molecular formula of C11H26N2O6, and a pKa of 6.8 at 20°C. Appearance: solid. Storage: room temperature.
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| Toxicity/Toxicokinetics |
Bis-tris propane is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use.
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| References | |
| Additional Infomation |
Di(trimethylolpropane) is a water-soluble buffer used in the preparation of biochemical and biological buffer solutions; its pKa is 6.8 at 20°C. It exhibits buffering activity, and its function is related to that of a member of the trimethylolpropane family.
Bis-tris propane has CAS number 64431-96-5, molecular formula C11H26N2O6, and molecular weight 282.33. Synonyms: 1,3-bis[tris(hydroxymethyl)methylamino]propane. It is a water-soluble buffer with a pKa of 6.8 at 20°C and a useful pH range of 6.3-9.5. Purity: ≥99%. Not for human use; for research purposes only. |
| Molecular Formula |
C11H26N2O6
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|---|---|
| Molecular Weight |
282.3339
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| Exact Mass |
282.179
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| CAS # |
64431-96-5
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| PubChem CID |
125132
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
573.3±50.0 °C at 760 mmHg
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| Melting Point |
164-165 °C(lit.)
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| Flash Point |
224.5±20.7 °C
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| Vapour Pressure |
0.0±3.6 mmHg at 25°C
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| Index of Refraction |
1.565
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| LogP |
-1.89
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
19
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| Complexity |
183
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HHKZCCWKTZRCCL-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
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| Chemical Name |
2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~354.20 mM)
DMSO : ~25 mg/mL (~88.55 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.85 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5420 mL | 17.7098 mL | 35.4195 mL | |
| 5 mM | 0.7084 mL | 3.5420 mL | 7.0839 mL | |
| 10 mM | 0.3542 mL | 1.7710 mL | 3.5420 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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