| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Biotin-PEG2-acid does not have a specific biological target, as it is a chemical linker. Its function is to serve as a covalent connector between molecular components. The biotin moiety has an exceptionally high affinity for the proteins streptavidin and avidin, with a dissociation constant (Kd) in the femtomolar range. This interaction is the basis for its use in detection and purification. The carboxylic acid group is a target for conjugation with primary amines, forming stable amide bonds. The hydrophilic PEG2 spacer enhances solubility and reduces steric hindrance between the conjugated molecules. Therefore, Biotin-PEG2-acid acts as a versatile connector, enabling the precise labeling and capture of biomolecules.
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| ln Vitro |
An ADC cytotoxin is connected to an antibody by use of an ADC linker to form an ADC. Two distinct ligands, one for the E3 ubiquitin ligase and the other for the target protein, are present in PROTAC and are joined by a linker. PROTAC targets and selectively degrades target proteins by means of the intracellular ubiquitin-proteasome system.
Biotin-PEG2-acid, as a linker, does not possess any direct in vitro biological activity, such as enzyme inhibition, receptor agonism, or cytotoxicity. Its function is to enable the synthesis of bioactive conjugates. The in vitro activity of this linker is assessed indirectly through the characterization of the final conjugates it helps to create. For instance, when used to synthesize a biotinylated antibody, the activity of the conjugate is evaluated in cell-based assays for target binding, internalization, and detection. The PEG2 linker contributes to the conjugate's overall activity by enhancing its solubility and providing the necessary spacing between the biotin and the conjugated molecule. Thus, the activity of Biotin-PEG2-acid is a measure of its utility as a synthetic building block. |
| ln Vivo |
Biotin-PEG2-acid is not intended for in vivo use as a standalone compound. Its relevance in vivo is as a component of larger conjugates, such as biotinylated antibodies or drug delivery systems. In these contexts, the hydrophilic PEG2 spacer contributes to the overall pharmacokinetic profile of the conjugate by improving solubility and reducing aggregation. However, the specific in vivo activity—such as target binding or therapeutic efficacy—is determined by the conjugate's targeting ligand and payload, not the linker itself. The linker's role is to stably connect the functional components and enable detection or purification.
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| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for Biotin-PEG2-acid, as it is not a biologically active compound. Its characterization is performed using analytical chemistry techniques such as NMR, HPLC, and MS to verify its structure and purity. The functionality of the biotin moiety can be confirmed by a simple streptavidin binding assay, such as a dot blot or an ELISA. The reactivity of the carboxylic acid can be tested by reacting it with a model amine and monitoring the formation of the amide bond by HPLC or LC-MS. These are quality control and functional validation steps, not biological assays.
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| Cell Assay |
There are no standard in vitro cell-based assays for Biotin-PEG2-acid itself, as it is not a bioactive molecule. Its use in cell biology is indirect, as a reagent for modifying other molecules. For example, it can be used to create biotinylated antibodies or fluorescent probes for cellular imaging or pull-down experiments. In such cases, the cell-based assay would involve treating cells with the final conjugated probe and then measuring a biological readout, such as fluorescence intensity, protein localization, or the identification of binding partners. The performance of the linker would be evaluated by the efficiency and specificity of the bioconjugation and the functionality of the probe. However, there is no direct assay for the linker itself in cells.
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| Animal Protocol |
Biotin-PEG2-acid is not used in in vivo animal experiments as a standalone compound. It is a synthetic building block for creating bioconjugates such as biotinylated antibodies or drug delivery systems. Animal studies would only be conducted on the final, larger conjugates that incorporate this linker. In such studies, the linker's contribution to the overall pharmacokinetic and pharmacodynamic profile of the conjugate might be assessed by comparing it to conjugates made with different linkers. However, there is no standard animal protocol for the linker itself. Its use is limited to the research and development phase, where it is employed in the chemical synthesis of test articles.
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| ADME/Pharmacokinetics |
Biotin-PEG2-acid has a molecular weight of 403.49 g/mol and a molecular formula of C17H29N3O6S. Its IUPAC name is 3-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]propanoic acid. It is a solid compound with a purity of >98.0%. It has a density of 1.2±0.1 g/cm³. It is soluble in DMSO at 10 mM. For storage, it is recommended to keep the powder at -20°C for up to 3 years or in solvent at -80°C for up to 2 years. The compound can be shipped at room temperature or with blue ice upon request.
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| Toxicity/Toxicokinetics |
Biotin-PEG2-acid is considered to have low toxicity, consistent with other biotin and PEG-based reagents. Biotin is a vitamin and is generally recognized as safe (GRAS). PEG polymers are widely regarded as biocompatible, non-toxic, and non-immunogenic. The compound is classified as a research reagent, and standard laboratory safety precautions should be followed when handling it. Its toxicity profile has not been extensively studied, but due to its intended use and chemical class, it is not expected to be acutely toxic. All handling should be done with appropriate personal protective equipment (PPE) in a well-ventilated area, and the compound should be stored properly to prevent degradation.
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| References | |
| Additional Infomation |
Biotin-PEG2-acid is a research-use-only reagent and is not a drug, nor is it approved for any clinical or therapeutic use. It is a heterobifunctional linker that combines a biotin moiety for high-affinity streptavidin binding with a terminal carboxylic acid for conjugation to amine-containing molecules. It serves as a functional linker enabling biotinylation of antibodies, proteins, or other biomolecules. The hydrophilic PEG2 spacer enhances water solubility and reduces steric hindrance. It is used in the preparation of antibody-drug conjugates (ADCs) and PROTAC protein degraders. Biotin-PEG2-acid is a valuable tool for bioconjugation, enabling the creation of stable, well-defined conjugates for research applications.
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| Molecular Formula |
C17H29N3O6S
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| Molecular Weight |
403.493663549423
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| Exact Mass |
403.177
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| CAS # |
1365655-89-5
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| PubChem CID |
60146201
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| Appearance |
Off-white to gray solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
752.2±60.0 °C at 760 mmHg
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| Flash Point |
408.7±32.9 °C
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| Vapour Pressure |
0.0±5.4 mmHg at 25°C
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| Index of Refraction |
1.527
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| LogP |
-1.05
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
27
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| Complexity |
507
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCOCCOCCC(=O)O)NC(=O)N2
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| InChi Key |
SDOAIZRTYJIBBG-XEZPLFJOSA-N
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| InChi Code |
InChI=1S/C17H29N3O6S/c21-14(18-6-8-26-10-9-25-7-5-15(22)23)4-2-1-3-13-16-12(11-27-13)19-17(24)20-16/h12-13,16H,1-11H2,(H,18,21)(H,22,23)(H2,19,20,24)/t12-,13-,16-/m0/s1
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| Chemical Name |
3-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~247.84 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.16 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4784 mL | 12.3919 mL | 24.7838 mL | |
| 5 mM | 0.4957 mL | 2.4784 mL | 4.9568 mL | |
| 10 mM | 0.2478 mL | 1.2392 mL | 2.4784 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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