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Purity: ≥98%
Biocytin is a conjugated adduct of L-lysine and D-biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme. Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.
| ln Vitro |
With a variety of avidin-conjugated markers, Biocytin may be seen at both the light and electron microscopic levels because of its strong affinity for avidin. The appearance of dendritic and axonal arborizations can be seen with the application of biocytin[1]. With a vast dendritic arbor and small population of ON cone bipolar cells, the Biocytin wide-field bipolar cell in rabbit retina does not make contact with every cone within its dendritic field. Cone types were identified using antibodies against blue cone opsin and red-green cone opsin, after which bipolar cells were identified by selective absorption of Biocytin and labeling of the cones with peanut agglutinin. The Biocytin-labeled cells avoid cones that do not stain for blue cone opsin and make selective contact with cones whose outer segments do. A homolog of the blue cone bipolar cells previously identified in the retinas of primates, mice, and ground squirrels, the biocytin wide-field bipolar cell is an ON blue cone bipolar cell found in the retina of rabbits[3].
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| References |
[1]. Mishra A, et al. Improved neuronal tract tracing with stable biocytin-derived neuroimaging agents. ACS Chem Neurosci. 2010 Feb 17;1(2):129-38.
[2]. Roth G, et al. Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde, and intracellular biocytin labeling study. J Comp Neurol. 2004 Oct 4;478(1):35-61. [3]. MacNeil MA, et al. Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J Comp Neurol. 2008 Jan 1;506(1):6-15. |
| Molecular Formula |
C16H28N4O4S
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|---|---|
| Molecular Weight |
372.48
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| Exact Mass |
372.1831
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| CAS # |
576-19-2
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| Related CAS # |
576-19-2;
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| SMILES |
N[C@@H](CCCCNC(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(O)=O
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| Chemical Name |
N6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl)-L-lysine
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| Synonyms |
Biotinyl-L-lysineBiocytin
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (~134.24 mM)
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6847 mL | 13.4235 mL | 26.8471 mL | |
| 5 mM | 0.5369 mL | 2.6847 mL | 5.3694 mL | |
| 10 mM | 0.2685 mL | 1.3424 mL | 2.6847 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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