| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
| Targets |
Bicine does not have a specific biological target as a drug; it is a buffer agent used in biochemical and physiological research. Its role is to maintain stable pH conditions in experimental systems, which is essential for enzyme activity, protein stability, and cellular function. As a zwitterionic buffer, Bicine does not readily cross cell membranes and has minimal interaction with biological molecules, making it suitable for studying sensitive biological processes. It is a derivative of glycine with two hydroxyethyl groups.
|
|---|---|
| ln Vitro |
Bicine is not evaluated for in vitro biological activity as a drug; it is a buffer reagent. Its utility is assessed by its buffering capacity, pH stability, and lack of interference with biochemical assays. It is used in enzyme assays, protein purification, and electrophoresis to maintain optimal pH conditions. Its zwitterionic nature minimizes interactions with proteins and other biomolecules.
|
| ln Vivo |
Bicine is not evaluated for in vivo biological activity as a drug; it is a buffer reagent. It is used in physiological research to maintain pH conditions in experimental systems. It is not administered as a therapeutic agent and does not have pharmacological effects.
|
| Enzyme Assay |
There are no specific in vitro enzyme or receptor binding assays for Bicine as a drug target. As a buffer, its properties are characterized by pH measurement, pKa determination, and assessment of compatibility with biological assays. The compound is typically evaluated for its buffering capacity at various temperatures and ionic strengths. Its suitability for specific applications is determined by its lack of interference with assay components.
|
| Cell Assay |
There are no specific in vitro cellular assays for Bicine as a pharmacologically active compound. As a buffer, it is used in cell culture media and biochemical assays to maintain pH. Typical cell culture applications involve incorporating Bicine into media at concentrations of 10-100 mM to buffer pH in the 7.6-9.0 range. Cell viability and function are monitored to ensure the buffer does not interfere with experimental outcomes.
|
| Animal Protocol |
There are no specific in vivo animal studies for Bicine as a pharmacologically active compound. As a buffer, it is not administered as a drug. It may be used in physiological experiments as a buffer for perfusion solutions or injection vehicles. However, its use in vivo is limited due to its zwitterionic nature and potential osmotic effects at high concentrations.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Bicine are not relevant as it is not a drug. As a small zwitterionic molecule (MW 163.17), if administered, it would be distributed in extracellular fluid and excreted unchanged in urine. It does not cross cell membranes readily. Bicine is not metabolized to a significant extent and is not expected to accumulate in tissues.
|
| Toxicity/Toxicokinetics |
The toxicity profile of Bicine is generally considered low. As a buffer used in research, it is not intended for human therapeutic use. Standard safety data indicate that Bicine is relatively non-toxic at concentrations typically used in buffer solutions (10-100 mM). However, high concentrations may cause osmotic effects or local irritation. No specific genotoxicity or carcinogenicity data are reported.
|
| Additional Infomation |
N,N-bis(2-hydroxyethyl)glycine is a bis(octanoic acid) and a Goodyear buffer with a pKa of 8.35 at 20°C. It is the conjugate acid of [bis(2-hydroxyethyl)amino]acetate and also a tautomer of [bis(2-hydroxyethyl)ammonium]acetate.
Bicine (CAS 150-25-4) is a zwitterionic amino acid buffer agent belonging to the Good's buffer series, with a pKa of 8.35 at 20°C and a useful pH range of 7.6-9.0. It has a molecular formula of C6H13NO4 and a molecular weight of 163.17. Bicine is widely used in biochemical and physiological research for enzyme assays, protein studies, and cell culture due to its mild, non-interfering nature. It is preferred for low-temperature biochemical work and sensitive assays. The compound is available for research use only and is not intended for therapeutic applications. |
| Molecular Formula |
C6H13NO4
|
|---|---|
| Molecular Weight |
163.1717
|
| Exact Mass |
163.084
|
| CAS # |
150-25-4
|
| Related CAS # |
139-41-3 (mono-hydrochloride salt);17123-43-2 (hydrochloride salt)
|
| PubChem CID |
8761
|
| Appearance |
White to off-white solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
388.5±27.0 °C at 760 mmHg
|
| Melting Point |
190 °C (dec.)(lit.)
|
| Flash Point |
188.8±23.7 °C
|
| Vapour Pressure |
0.0±2.0 mmHg at 25°C
|
| Index of Refraction |
1.526
|
| LogP |
-0.47
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
11
|
| Complexity |
111
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O([H])C([H])([H])C([H])([H])N(C([H])([H])C(=O)O[H])C([H])([H])C([H])([H])O[H]
|
| InChi Key |
FSVCELGFZIQNCK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11)
|
| Chemical Name |
2-[bis(2-hydroxyethyl)amino]acetic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~166.67 mg/mL (~1021.45 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (306.43 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.1286 mL | 30.6429 mL | 61.2858 mL | |
| 5 mM | 1.2257 mL | 6.1286 mL | 12.2572 mL | |
| 10 mM | 0.6129 mL | 3.0643 mL | 6.1286 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
