| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
β-Glucuronidase-IN-1 (Inhibitor 1) (0.01-100 μM) suppresses the β-glucuronidase activity of E. coli in a dose-dependent manner with IC50 and Ki values of 283 nM and 164 nM, respectively [1]. β-Glucuronidase-IN-1 (100 μM; 24-72 hours) does not affect the development of bacterial cells in either aerobic or anaerobic settings while maintaining a strong efficacy in living bacterial cells (EC50=17.7 nM). won't destroy the epithelial cells in mammals[1].
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| ln Vivo |
β-Glucuronidase-IN-1 (oral gavage; 10 μg; twice daily; 11 days) preserves the glandular structure of tract tissue in CPT-11-treated intestines and shields the mouse gastrointestinal epithelium from damage[1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: CMT93, CaCO-2, HCT116 Cell Tested Concentrations: 100 μM Incubation Duration: 24 hrs (hours), 48 hrs (hours) and 72 hrs (hours) Experimental Results: Mammalian cell survival is not affected, any decrease in cell viability is attributed to presence of DMSO. |
| Animal Protocol |
Animal/Disease Models: Healthy 6- to 8weeks old Balb/cJ mice [1]
Doses: 10 μg Route of Administration: po (oral gavage); twice a day; 11 days Experimental Results: Reduces the toxicity of mice caused by CPT-11. |
| References |
[1]. Wallace BD, et al. Alleviating cancer drug toxicity by inhibiting a bacterial enzyme.Science. 2010 Nov 5;330(6005):831-5.
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| Molecular Formula |
C23H27N3O3S
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|---|---|
| Molecular Weight |
425.544
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| CAS # |
484006-66-8
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| SMILES |
CC1C=C(C)C=C2C=C(CN(CCO)C(=S)NC3C=CC(OCC)=CC=3)C(=O)NC=12
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~235.00 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (5.87 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3500 mL | 11.7498 mL | 23.4996 mL | |
| 5 mM | 0.4700 mL | 2.3500 mL | 4.6999 mL | |
| 10 mM | 0.2350 mL | 1.1750 mL | 2.3500 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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