| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
Benzyladenine targets cytokinin receptors in plants, mimicking the natural cytokinin zeatin. It activates P2-purinergic receptors inducing positive inotropic effects. As a plant growth regulator, it promotes cell division, shoot proliferation, and delays senescence. Benzyladenine inhibits respiratory kinase in plants. Its mechanism of action involves the activation of cytokinin signaling pathways, leading to enhanced plant growth and development. Benzyladenine is not used as a therapeutic agent in humans.
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| ln Vitro |
In vitro, benzyladenine is a plant growth regulator that stimulates cell division and shoot proliferation in plant tissue culture. It is used in plant tissue culture media to promote shoot regeneration and multiplication. Benzyladenine activates P2-purinergic receptors inducing positive inotropic effects. It inhibits respiratory kinase in plants. The compound's effects on plant cells are assessed using standard plant tissue culture techniques. Benzyladenine is not used in mammalian cell-based assays.
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| ln Vivo |
In vivo, benzyladenine is used in agriculture and horticulture to promote plant growth and development. It is applied to crops such as apples, melons, oranges, roses, pears, sweet cherries, and white pine trees to stimulate growth. Benzyladenine increases the post-harvest life of green vegetables. It is not used as a therapeutic agent in animals or humans. The compound is for agricultural use only and is not a pharmaceutical agent.
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| Enzyme Assay |
In vitro enzyme assays for benzyladenine are not typical, as the compound is a plant growth regulator rather than a pharmaceutical agent. Its activity is assessed using plant-based assays, such as measuring cytokinin activity in plant tissue culture. The compound's ability to activate cytokinin receptors can be assessed using plant bioassays measuring shoot proliferation or chlorophyll retention. These assays confirm the compound's mechanism as a cytokinin analog.
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| Cell Assay |
In vitro cell-based assays for benzyladenine are not typically performed in mammalian systems, as the compound is a plant growth regulator. However, its effects on P2-purinergic receptors have been studied in mammalian cells. Cells expressing P2-purinergic receptors are treated with benzyladenine, and receptor activation is assessed by measuring downstream signaling. These studies are limited and the compound is primarily used in plant research.
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| Animal Protocol |
In vivo animal experiments for benzyladenine are not typically conducted, as the compound is a plant growth regulator rather than a therapeutic agent. It is not administered to animals for pharmacological purposes. The compound is for agricultural use only and is not a pharmaceutical agent. Comprehensive in vivo studies in animals are limited.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
This report summarizes the metabolic results of the labeled compound in rats and dogs. In the canine experiments, the compound (15 mg, labeled benzyl-1-C14-adenine) was mixed into a small piece of meat and fed to the animal. Urine was collected at 24 and 48 hours. Almost all radioactive material was excreted in the urine within 24 hours. Three metabolites were identified by co-chromatography: hippuric acid, benzoic acid, and benzyladenine (same as in rats). For animals given benzyladenine-8-C14, the metabolic profile was slightly different, and no other components besides the parent compound were identified. The excretion rate of the adenine label was slower. The metabolic profiles in rats and dogs were again similar. [California Environmental Protection Agency/Pesticide Management Department; Summary of Toxicological Data for Cytokinin and 6-Benzyladenine] Pharmacokinetic (PK) data for benzyladenine are not relevant, as the compound is a plant growth regulator rather than a therapeutic agent. It has a molecular weight of 225.26 g/mol and is a white to off-white or yellow powder with a melting point of 230-233°C. It is for agricultural use only and is not administered to humans or animals for therapeutic purposes. |
| Toxicity/Toxicokinetics |
Toxicity Data
LC50 (Rabbit) = 5,200 mg/m³/1hr Non-human Toxicity Values Rabbit Dermal LD50: 5 g/kg Rabbit Inhalation LC50: 5.2 mg/L 1 hour Rat Oral LD50: 1.3 g/kg The toxicity of benzyladenine to mammals is low. It is used in agriculture as a plant growth regulator and is not intended for human or veterinary use. The compound should be handled with appropriate safety precautions. For research and agricultural use only. |
| References | |
| Additional Infomation |
N-Benzyladenine belongs to the 6-aminopurine class of compounds, and its structure is formed by replacing one amino hydrogen atom of adenine with a benzyl group. It is a plant metabolite and cytokinin, functionally related to adenine. 6-Benzylaminopurine has been reported to be present in hops (Humulus lupulus), rehmannia (Rehmannia glutinosa), and several other organisms with relevant data.
Benzyladenine (6-Benzylaminopurine) is a synthetic compound that mimics the natural cytokinin, zeatin, and is widely used in agriculture and horticulture to promote plant growth and development. It is a plant growth regulator used to stimulate growth in various crops. Benzyladenine is an inhibitor of respiratory kinase in plants and increases the post-harvest life of green vegetables. It is not a pharmaceutical agent and has no clinical applications. |
| Molecular Formula |
C12H11N5
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|---|---|
| Molecular Weight |
225.2492
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| Exact Mass |
225.101
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| CAS # |
1214-39-7
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| Related CAS # |
6-Benzylaminopurine-d5;2322358-20-1
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| PubChem CID |
62389
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
387.5±52.0 °C at 760 mmHg
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| Melting Point |
230-233 °C
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| Flash Point |
188.1±30.7 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.731
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| LogP |
0.28
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
17
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| Complexity |
241
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NWBJYWHLCVSVIJ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C12H11N5/c1-2-4-9(5-3-1)6-13-11-10-12(15-7-14-10)17-8-16-11/h1-5,7-8H,6H2,(H2,13,14,15,16,17)
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| Chemical Name |
N-benzyl-7H-purin-6-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~221.98 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4395 mL | 22.1976 mL | 44.3951 mL | |
| 5 mM | 0.8879 mL | 4.4395 mL | 8.8790 mL | |
| 10 mM | 0.4440 mL | 2.2198 mL | 4.4395 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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