| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
|
||
| Other Sizes |
| Targets |
Benzylacetone targets mushroom tyrosinase (IC₅₀: 0.52 mM, determined by tyrosinase activity assay) [2]
|
|---|---|
| ln Vitro |
Benzylacetone inhibited the activity of mushroom tyrosinase in a dose-dependent manner, with an IC₅₀ value of 0.52 mM [2]
It suppressed tyrosinase-mediated L-tyrosine oxidation, reducing the formation of dopachrome (the reaction product) [2] |
| ln Vivo |
In ICR mice, inhalation of Benzylacetone exhibited appetite-enhancing effects, leading to increased body weight compared to the control group [3]
The effect was concentration- and time-dependent: significant weight gain was observed at an inhalation concentration of 10 ppm, with a 30-minute daily inhalation duration for 14 consecutive days [3] Combined inhalation of Benzylacetone (10 ppm) and trans-cinnamaldehyde (10 ppm) showed a synergistic effect on appetite enhancement and weight gain [3] |
| Enzyme Assay |
Mushroom tyrosinase was dissolved in phosphate buffer (pH 6.8) to prepare an enzyme solution [2]
Benzylacetone was dissolved in ethanol (final ethanol concentration < 1%) and serially diluted to concentrations ranging from 0.1 to 2 mM [2] The reaction mixture contained enzyme solution, Benzylacetone solution, and L-tyrosine (substrate) in phosphate buffer, incubated at 37°C for 30 minutes [2] The absorbance of dopachrome was measured at 475 nm to assess tyrosinase activity [2] The inhibition rate was calculated relative to the control group (without Benzylacetone), and the IC₅₀ value was determined by fitting the dose-response curve [2] |
| Animal Protocol |
Male ICR mice (4 weeks old) were randomly divided into control group, Benzylacetone single-inhalation groups (5, 10, 20 ppm), and combined-inhalation group (10 ppm Benzylacetone + 10 ppm trans-cinnamaldehyde) (n=6 per group) [3]
Mice were placed in inhalation chambers, and Benzylacetone vapor was generated by heating the liquid compound to the desired concentration, controlled by air flow rate [3] Inhalation was performed once daily for 14 consecutive days, with durations of 15, 30, or 60 minutes per day [3] Body weight was measured every 2 days, and food intake was recorded daily throughout the experiment [3] At the end of the experiment, no obvious toxicity signs were observed, and mice were euthanized for further analysis [3] |
| References |
|
| Additional Infomation |
4-Phenylacet-2-one is a ketone. Benzylacetone has been reported to be found in Alpinia latilabris, Rhododendron anthopogonaides, and other organisms with relevant data. Benzylacetone is a low molecular weight aromatic compound [1]. Literature [1] mentions that it is a heated derivative of agarwood [1]. Its tyrosinase inhibitory activity suggests potential applications in cosmetics (e.g., skin whitening) [2]. It enhances appetite upon inhalation, suggesting potential use in treating anorexia, and has a synergistic effect when used in combination with trans-cinnamaldehyde [3].
|
| Molecular Formula |
C10H12O
|
|---|---|
| Molecular Weight |
148.2017
|
| Exact Mass |
148.088
|
| CAS # |
2550-26-7
|
| PubChem CID |
17355
|
| Appearance |
Colorless to light yellow liquid
|
| Density |
1.0±0.1 g/cm3
|
| Boiling Point |
233.5±0.0 °C at 760 mmHg
|
| Melting Point |
-13ºC
|
| Flash Point |
98.3±0.0 °C
|
| Vapour Pressure |
0.1±0.4 mmHg at 25°C
|
| Index of Refraction |
1.504
|
| LogP |
1.67
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
11
|
| Complexity |
123
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O=C(C([H])([H])[H])C([H])([H])C([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H]
|
| InChi Key |
AKGGYBADQZYZPD-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C10H12O/c1-9(11)7-8-10-5-3-2-4-6-10/h2-6H,7-8H2,1H3
|
| Chemical Name |
4-phenylbutan-2-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~674.72 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.87 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (16.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7476 mL | 33.7382 mL | 67.4764 mL | |
| 5 mM | 1.3495 mL | 6.7476 mL | 13.4953 mL | |
| 10 mM | 0.6748 mL | 3.3738 mL | 6.7476 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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