| Size | Price | Stock | Qty |
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| 500mg |
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Purity: ≥98%
Benfluorex (Mediator) is an anorectic and hypolipidemic agent that is structurally related to fenfluramine. It may improve glycemic control and decrease insulin resistance in people with poorly controlled type-2 diabetes.
| ln Vitro |
Benfluex hydrochloride increases the amount of GFP-positive cells, a sustainable way to stimulate insulin promoter activity. In a dose-dependent manner, benfluex hydrochloride raises endogenous insulin mRNA levels and the quantity of GFP-positive cells. Benfluex hydrochloride increases the expression of HNF4α, which is consistent with its role as an activator of HNF4α. HNF4α protease's sensitivity is altered by benfluex hydrochloride, but not by an inert control drug [1]. In oleic acid, benfluex hydrochloride decreases the synthesis of ketone bodies and acid lysates in a concentration-dependent manner, but it dramatically increases the amount of 14CO2 that enters the citric acid cycle. In a dose-dependent way, benfluex hydrochloride reduces the rate of gluconeogenesis from lactate/pyruvate (10/1 nM) [2].
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| References |
[1]. Lee SH, et al. Identification of alverine and benfluorex as HNF4α activators. ACS Chem Biol. 2013 Aug 16;8(8):1730-6.
[2]. Kohl C, et al. Effects of benfluorex on fatty acid and glucose metabolism in isolated rat hepatocytes: from metabolic fluxes to gene expression. Diabetes. 2002 Aug;51(8):2363-8 |
| Molecular Formula |
C19H21CLF3NO2
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|---|---|
| Molecular Weight |
387.82374
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| CAS # |
23642-66-2
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| Related CAS # |
Benfluorex;23602-78-0
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| SMILES |
O=C(OCCNC(C)CC1=CC=CC(C(F)(F)F)=C1)C2=CC=CC=C2.[H]Cl
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| InChi Key |
NLOALSPYZIIXEO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H20F3NO2.ClH/c1-14(12-15-6-5-9-17(13-15)19(20,21)22)23-10-11-25-18(24)16-7-3-2-4-8-16/h2-9,13-14,23H,10-12H2,1H31H
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| Chemical Name |
2-((1-(3-(trifluoromethyl)phenyl)propan-2-yl)amino)ethyl benzoate hydrochloride
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| Synonyms |
Benfluorex hydrochloride Lipophoral Mediator JP 992 EINECS 245-801-8 Mediaxal Balans EINECS 245-020-2
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~257.85 mM)
H2O : ~2.27 mg/mL (~5.85 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 5.88 mg/mL (15.16 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5785 mL | 12.8926 mL | 25.7852 mL | |
| 5 mM | 0.5157 mL | 2.5785 mL | 5.1570 mL | |
| 10 mM | 0.2579 mL | 1.2893 mL | 2.5785 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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