| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
BCA Disodium does not have a biological target in the traditional sense of a receptor or enzyme. Its primary function is as a chemical reagent. It acts as a chelating agent that specifically binds to cuprous ions (Cu⁺) to form a stable, purple-colored complex. This property is exploited in the BCA protein assay, where it serves as the detection reagent for the Cu⁺ ions generated by the reduction of Cu²⁺ by proteins in an alkaline medium.
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|---|---|
| ln Vitro |
In vitro, BCA Disodium is used to quantify protein concentration. The assay relies on the reduction of Cu²⁺ to Cu⁺ by proteins in an alkaline environment (biuret reaction). The BCA reagent then chelates the Cu⁺ ions, forming a purple-colored complex that exhibits a strong absorbance at 562 nm. The absorbance is directly proportional to the protein concentration in the sample, allowing for accurate and sensitive protein quantification.
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| ln Vivo |
BCA Disodium is not used for in vivo applications. Its utility is entirely confined to in vitro biochemical assays for research and diagnostic purposes. There are no known in vivo activities for this compound, as its function is strictly as an analytical reagent for protein and copper detection.
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| Enzyme Assay |
The in vitro enzyme/receptor binding assay protocol for BCA Disodium is the standard BCA protein assay. In this assay, a working reagent (WR) is prepared by mixing BCA Reagent A (which contains BCA Disodium, sodium carbonate, sodium tartrate, and sodium bicarbonate) with Reagent B (4% copper sulfate). The sample or standard is mixed with the WR and incubated at 37°C or 60°C. The reduction of Cu²⁺ to Cu⁺ by the protein, followed by the formation of the BCA-Cu⁺ complex, produces a color change measured at 562 nm.
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| Cell Assay |
The in vitro cellular assay using BCA Disodium is not applicable in the context of a drug compound. However, BCA Disodium can be used to quantify the protein content of cell lysates. In this application, cells are lysed, and the lysate is mixed with the BCA working reagent. After incubation, the absorbance is measured at 562 nm, and the protein concentration is determined by comparing to a standard curve, typically using bovine serum albumin (BSA) as a standard.
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| Animal Protocol |
BCA Disodium is not used in in vivo animal experiments, as it is a reagent for in vitro use only. There are no established protocols for its administration to animals for any therapeutic or experimental purpose, as its function is purely analytical and not biological.
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| ADME/Pharmacokinetics |
As a chemical reagent, BCA Disodium does not have pharmacokinetic (PK) properties in the context of drug development. It is not administered systemically for therapeutic effects. However, its chemical stability is well-characterized. It is a stable, water-soluble compound. The compound should be protected from light and stored in a sealed, dry environment to prevent degradation. Its solubility in water is high (~100 mg/mL), which is essential for its use in aqueous assay buffers.
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| Toxicity/Toxicokinetics |
Toxicological data for BCA Disodium are not typically reported as it is not a therapeutic agent. Standard laboratory safety precautions should be followed when handling it. The material safety data sheet (MSDS) would classify it as a non-hazardous chemical, but it is recommended to avoid inhalation, ingestion, and skin contact. As with any chemical reagent, appropriate personal protective equipment (PPE) should be used.
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| References |
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| Additional Infomation |
BCA Disodium is also known as bicinchoninic acid disodium salt, 2,2'-biquinoline-4,4'-dicarboxylic acid disodium salt, and is commonly referred to as BCA-2Na. It is a fundamental reagent in biochemistry and molecular biology laboratories for protein quantification. Its sensitivity is similar to that of neocuproine for copper detection. It is not a drug and has no clinical applications or approvals. The compound is exclusively for research use.
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| Molecular Formula |
C20H10N2NA2O4
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|---|---|
| Molecular Weight |
388.2839
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| Exact Mass |
388.043
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| CAS # |
979-88-4
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| PubChem CID |
164763
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| Appearance |
White to light yellow solid powder
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| Boiling Point |
530.6ºC at 760 mmHg
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| Flash Point |
274.7ºC
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| LogP |
1.177
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
28
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| Complexity |
495
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
AUPXFICLXPLHBB-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C20H12N2O4.2Na/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);;/q;2*+1/p-2
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| Chemical Name |
disodium;2-(4-carboxylatoquinolin-2-yl)quinoline-4-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~257.55 mM)
DMSO : ~2 mg/mL (~5.15 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (64.39 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5755 mL | 12.8773 mL | 25.7546 mL | |
| 5 mM | 0.5151 mL | 2.5755 mL | 5.1509 mL | |
| 10 mM | 0.2575 mL | 1.2877 mL | 2.5755 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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