| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| Other Sizes |
|
| ln Vitro |
Bax-dependent tumor cell death is induced in a dose-dependent manner by Bax activator-1 (20-80 μM; 48 hours) [1].
|
|---|---|
| ln Vivo |
In C57BL/6 female mice, the intraperitoneal injection of Bax activator-1 (40 mg/kg) suppresses the formation of lung tumors every day for 13 days [1].
|
| Cell Assay |
Apoptosis analysis [1]
Cell Types: Lewis lung cancer cells, human non-small cell lung cancer A549 cells, human pancreatic cancer Panc-1 cells Tested Concentrations: 20μM, 40μM, 60μM, 80μM Incubation Duration: 48 hrs (hours) Experimental Results: In a dose-dependent manner Induces apoptosis in mouse Lewis lung cancer (LLC), human non-small cell lung cancer A549 cells and PANC-1 human pancreatic cancer cells. |
| Animal Protocol |
Animal/Disease Models: Eightweeks old C57BL/6 female mice (containing Lewis lung cancer tumor cells) [1]
Doses: 40 mg/kg Route of Administration: daily intraperitoneal (ip) injection for 13 days Experimental Results: demonstrated anti-tumor activity as a single agent agent. |
| References |
| Molecular Formula |
C29H36N4O3
|
|---|---|
| Molecular Weight |
488.621147155762
|
| Exact Mass |
488.278
|
| CAS # |
1638526-94-9
|
| PubChem CID |
26230248
|
| Appearance |
White to off-white solid powder
|
| LogP |
3.6
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
36
|
| Complexity |
737
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
O[C@@H]1CCCN(C(C2C3CN(CC4C=CC(C)=C(C)C=4)CCC=3N(CC3C=CC=C(C=3)OC)N=2)=O)C1
|
| InChi Key |
OLNVSYZSZXRLCF-XMMPIXPASA-N
|
| InChi Code |
InChI=1S/C29H36N4O3/c1-20-9-10-23(14-21(20)2)16-31-13-11-27-26(19-31)28(29(35)32-12-5-7-24(34)18-32)30-33(27)17-22-6-4-8-25(15-22)36-3/h4,6,8-10,14-15,24,34H,5,7,11-13,16-19H2,1-3H3/t24-/m1/s1
|
| Chemical Name |
[5-[(3,4-dimethylphenyl)methyl]-1-[(3-methoxyphenyl)methyl]-6,7-dihydro-4H-pyrazolo[4,3-c]pyridin-3-yl]-[(3R)-3-hydroxypiperidin-1-yl]methanone
|
| Synonyms |
Bax-activator-106 Bax activator 106 Baxactivator106
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~511.65 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.26 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.26 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (4.26 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0466 mL | 10.2329 mL | 20.4658 mL | |
| 5 mM | 0.4093 mL | 2.0466 mL | 4.0932 mL | |
| 10 mM | 0.2047 mL | 1.0233 mL | 2.0466 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
