| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| 10g |
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| Other Sizes |
Purity: ≥98%
| Targets |
Bacterial
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|---|---|
| ln Vitro |
An antibiotic called azomycin has the ability to combat both aerobic Gram-positive and Gram-negative bacteria. Azomycin has been shown to be effective against a range of anaerobic bacteria[1].
azomycin was found to be active against a variety of anaerobic bacteria. The minimum inhibitory concentrations (MICs) against tested anaerobic strains are as follows: Peptococcus asaccharolyticus ATCC14963: 3.13 μg/ml; P. prevotii ATCC9321: 6.25 μg/ml; Peptostreptococcus micros VPI 5464-1: 1.56 μg/ml; Streptococcus constellatus ATCC27823: >100 μg/ml; Eubacterium limosum ATCC8486: 3.13 μg/ml; E. aerofaciens ATCC25986: 6.25 μg/ml; Propionibacterium acnes ATCC11827: 50 μg/ml; Bifidobacterium adolescentis JCM1250: 6.25 μg/ml; B. bifidum JCM1122: 6.25 μg/ml; B. longum ATCC15707: 12.5 μg/ml; Clostridium perfringens ATCC13124: 0.78 μg/ml; C. difficile ATCC17857: 0.78 μg/ml; Veillonella parvula ATCC10790: 1.56 μg/ml; Bacteroides fragilis GM7000: 3.13 μg/ml; B. fragilis ATCC25285: 3.13 μg/ml; B. thetaiotaomicron WAL3304: 6.25 μg/ml; B. vulgatus ATCC29327: 0.39 μg/ml; B. melaninogenicus GAI0413: 0.39 μg/ml; Fusobacterium varium ATCC8501: 1.56 μg/ml; F. necrophorum ATCC25286: 0.78 μg/ml; F. nucleatum ATCC25586: 1.56 μg/ml; F. mortiferum ATCC9817: 0.78 μg/ml. [1] It is mentioned that azomycin has been previously reported to be active against aerobic Gram-positive and Gram-negative bacteria. [1] |
| Cell Assay |
The antibacterial activity of azomycin against anaerobic bacteria was evaluated using an agar dilution method. The test medium was GAM agar. The inoculum size was one loopful of a bacterial suspension adjusted to 10^6 colony-forming units per milliliter (cfu/ml). The minimum inhibitory concentration (MIC) was determined as the lowest concentration of azomycin that prevented visible growth after incubation. [1]
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| References | |
| Additional Infomation |
2-Nitroimidazole is an imidazole compound with a nitro group substituted at the 2-position of 1H-imidazole. It possesses anti-tuberculosis activity. 2-Nitroimidazole is a C-nitro compound belonging to the imidazole class. Its function is similar to that of 1H-imidazole. 2-Nitroimidazole has been reported to exist in Pseudomonas fluorescens, and relevant data are available for reference.
Azomycin (2-nitroimidazole) was isolated from Pseudomonas fluorescens PB-6282. The fermentation medium contained starch 2.0%, glycerol 0.5%, Bacto Soytone 1.5%, corn steep liquor 0.5%, NaCl 0.3%, CaCO3 0.3% (pH 7.0). The culture was incubated at 23°C for 2 days on a rotary shaker (180 rpm, 70 mm stroke). The isolation procedure involved pH adjustment to 2.0 with HCl, extraction with BuOH, back-extraction into aqueous NaHCO3, re-extraction at pH 2.0, followed by Sephadex LH-20 column chromatography with MeOH, silica gel column chromatography with CHCl3-MeOH (20:1), and crystallization from MeOH to yield colorless prisms. [1] The UV absorption spectrum of azomycin in 95% EtOH showed λmax (ε) 219 (3,900) and 315 (8,600); in 0.1 N HCl-95% EtOH: 219 (3,900) and 315 (8,400); in 0.1 N NaOH-95% EtOH: 223 (3,500) and 365 (10,700). The IR spectrum (KBr) was consistent with reported data for azomycin. No optical activity was detected by CD measurement. 1H NMR (DMSO-d6, TMS): δ 7.41 (s, 4-H, 5-H); 13C NMR: δ 126.3 (d, 1JCH=195 Hz, long range coupling 2JCH=12 Hz, C-4 and C-5), δ 146.1 (s, C-2). EI-MS: m/z 113 (M+). Elemental analysis: Calcd for C3H3N3O2: C 31.86, H 2.67, N 37.16; Found: C 31.86, H 2.92, N 37.10. [1] |
| Molecular Formula |
C3H3N3O2
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|---|---|---|
| Molecular Weight |
113.07
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| Exact Mass |
113.022
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| Elemental Analysis |
C, 31.87; H, 2.67; N, 37.16; O, 28.30
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| CAS # |
527-73-1
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| Related CAS # |
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| PubChem CID |
10701
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| Appearance |
White to yellow solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
373.6±25.0 °C at 760 mmHg
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| Melting Point |
287 °C (dec.)(lit.)
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| Flash Point |
179.7±23.2 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.612
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| LogP |
0.15
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
8
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| Complexity |
99.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=[N+]([O-])C1=NC=CN1
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| InChi Key |
YZEUHQHUFTYLPH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C3H3N3O2/c7-6(8)3-4-1-2-5-3/h1-2H,(H,4,5)
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| Chemical Name |
2-nitro-1H-imidazole
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| Synonyms |
Amicin; 2-Nitroimidazole;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 22~50 mg/mL ( 194.56~442.20 mM )
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.67 mg/mL (23.61 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 26.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.67 mg/mL (23.61 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 26.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. View More
Solubility in Formulation 3: 10% DMSO+40% PEG300+5% Tween-80+45% Saline: ≥ 2.67 mg/mL (23.61 mM) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.8441 mL | 44.2204 mL | 88.4408 mL | |
| 5 mM | 1.7688 mL | 8.8441 mL | 17.6882 mL | |
| 10 mM | 0.8844 mL | 4.4220 mL | 8.8441 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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