| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 25mg |
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| 1g |
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| Other Sizes |
Purity: ≥98%
Azido-PEG4-alcohol is a PEG analogue containing an azide group and a terminal hydroxyl group. The hydrophilic PEG spacer increases solubility in aqueous media. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The hydroxyl group enables further derivatization or replacement with other reactive functional groups.
| Targets |
Azido-PEG4-alcohol itself does not have a specific biological target. It is a chemical linker and does not directly bind to any biological target. The reactive target of its azide group is other chemical groups, such as alkyne, DBCO, or BCN.
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|---|---|
| ln Vitro |
One ligand is for an E3 ubiquitin ligase, and the other is for the target protein; these two ligands are joined by a linker to form PROTACs. The intracellular ubiquitin-proteasome system is utilized by PROTACs to specifically destroy target proteins[1].
As a standalone compound, it has no direct in vitro biological activity. Its "activity" is reflected in the efficiency of Click Chemistry reactions; its azide group can rapidly and efficiently undergo cycloaddition with alkyne groups, with or without a copper catalyst, to form a stable triazole linkage. This reaction is highly specific (bioorthogonal) under physiological conditions. |
| Enzyme Assay |
Azido-PEG4-alcohol does not directly bind to enzymes or receptors, therefore no standard protocol exists for such assays. Its application in in vitro chemical synthesis involves mixing the compound (dissolved in an organic solvent like DMSO or DMF) with an alkyne-containing molecule and a copper catalyst (e.g., CuSO₄/sodium ascorbate) for several hours to overnight.
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| Toxicity/Toxicokinetics |
Safety Data Sheets (SDS) indicate that Azido-PEG4-alcohol in its concentrated form is a skin, eye, and respiratory tract irritant (H315, H319, H335). Azides also pose a potential explosive decomposition risk when heated or exposed to reducing agents or strong acids. For toxicity and pharmacology, the data of the complete PROTAC drug molecule are clinically relevant.
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| References |
| Molecular Formula |
C8H17N3O4
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|---|---|
| Molecular Weight |
219.23828
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| Exact Mass |
219.122
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| CAS # |
86770-67-4
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| Related CAS # |
86770-67-4;
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| PubChem CID |
4614742
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| Appearance |
Colorless to light yellow liquid
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| Index of Refraction |
1.47
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| LogP |
0
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
15
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| Complexity |
173
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[N-]=[N+]=NCCOCCOCCOCCO
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| InChi Key |
MBQYGQMGPFNSAP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H17N3O4/c9-11-10-1-3-13-5-7-15-8-6-14-4-2-12/h12H,1-8H2
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| Chemical Name |
2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethanol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~456.12 mM)
DMSO : ~33.33 mg/mL (~152.03 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.40 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.40 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.40 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (456.12 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5612 mL | 22.8061 mL | 45.6121 mL | |
| 5 mM | 0.9122 mL | 4.5612 mL | 9.1224 mL | |
| 10 mM | 0.4561 mL | 2.2806 mL | 4.5612 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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