| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
AV-153 targets DNA, intercalating into single-strand breaks. This interaction appears to stimulate DNA repair mechanisms. It also influences poly(ADP)ribosylation, a process involved in DNA repair and cellular stress responses. These activities contribute to its antimutagenic and anticancer properties.
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| ln Vitro |
Ava-153 free base (1 nM to 10 μM; 3 hours) decreases spontaneously generated DNA single-strand breaks (SSB) levels in peripheral blood lymphocytes or HL-60 cells by 13–67% [1]. The IC50 values of AV-153 free base on Raji and HL-60 cells are 14.9 mM and 10.3 mM, respectively[1].
In vitro, AV-153 free acid intercalates into DNA at single-strand breaks, reducing DNA damage and stimulating DNA repair in human cells. It interacts with thymine and cytosine and has an influence on poly(ADP)ribosylation. It exhibits anticancer activity. |
| ln Vivo |
In vivo, AV-153 free acid is a research compound with potential anticancer activity. Its ability to stimulate DNA repair suggests it could protect cells from mutagenic insults. However, specific in vivo efficacy data are not detailed in the search results. It is a tool for studying DNA repair and mutagenesis.
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| Enzyme Assay |
In vitro assays for AV-153 involve studying its interaction with DNA. This can be done using DNA binding assays, such as UV-Vis spectroscopy, fluorescence spectroscopy, or gel electrophoresis to study intercalation. The stimulation of DNA repair can be measured by assessing the repair of damaged DNA in cell-based assays.
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| Cell Assay |
In vitro cellular studies are conducted using human cells in culture. Cells are treated with AV-153, and DNA damage is induced (e.g., by UV radiation or chemicals). The compound's ability to reduce DNA damage and stimulate repair is assessed by measuring DNA strand breaks (comet assay) or by quantifying the repair of specific lesions.
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| Animal Protocol |
In vivo animal experiments are not detailed in the search results. However, as a potential anticancer agent, it could be studied in xenograft models. Animals would be administered AV-153, and its effect on tumor growth and the extent of DNA damage in tumor tissues would be assessed.
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| ADME/Pharmacokinetics |
AV-153 free acid has a molecular formula of C14H19NO6 and a molecular weight of 297.30 g/mol. It is a 1,4-dihydropyridine derivative. It appears as a white to light yellow solid powder. It is typically stored at -20°C. Its purity is high for research-grade material (≥97%).
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| Toxicity/Toxicokinetics |
As a research chemical, AV-153 free acid is not intended for human use. Its toxicological profile is not fully characterized. Standard laboratory safety precautions should be followed when handling it. Its antimutagenic properties suggest it could be protective, but this is for research purposes.
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| References |
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| Additional Infomation |
AV-153 free acid is also known as AV-153 free base and has the related CAS number 27296-05-5. It is a 1,4-dihydropyridine derivative with antimutagenic and anticancer activity. It is a valuable tool for studying DNA repair mechanisms and the effects of DNA damage. It is supplied as a high-purity research compound.
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| Molecular Formula |
C₁₄H₁₉NO₆
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|---|---|
| Molecular Weight |
297.30
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| Exact Mass |
297.121
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| CAS # |
19350-66-4
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| Related CAS # |
AV-153;27296-05-5
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| PubChem CID |
168683
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| Appearance |
White to light yellow solid powder
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| Density |
1.23g/cm3
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| Boiling Point |
450.5ºC at 760mmHg
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| Flash Point |
226.3ºC
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| Vapour Pressure |
2.26E-09mmHg at 25°C
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| Index of Refraction |
1.51
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| LogP |
1.293
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
21
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| Complexity |
492
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
USGQSNVLGQWBEC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H19NO6/c1-5-20-13(18)9-7(3)15-8(4)10(11(9)12(16)17)14(19)21-6-2/h11,15H,5-6H2,1-4H3,(H,16,17)
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| Chemical Name |
3,5-bis(ethoxycarbonyl)-2,6-dimethyl-1,4-dihydropyridine-4-carboxylic acid
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| Synonyms |
AV 153; AV-153; AV153
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~840.90 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3636 mL | 16.8180 mL | 33.6361 mL | |
| 5 mM | 0.6727 mL | 3.3636 mL | 6.7272 mL | |
| 10 mM | 0.3364 mL | 1.6818 mL | 3.3636 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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