| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
ATP disodium salt (5mM; 1 hour) combined with LPS (1 μg/ml) enhances the activation of the NLRP3 inflammasome in HGFs [3]. In vitro, ATP disodium salt (2 mM; 0.5-24 hours) stimulates the production of interleukin 1β, KC, and MIP-2 from bone marrow-derived macrophages (BMDMs) via caspase-1 activation [4]. ATP disodium salt stimulates cytokine and chemokine release, as well as inflammasome activation, which both directly and indirectly induce neutrophil chemotaxis in vitro[4].
|
|---|---|
| ln Vivo |
In vivo protection against bacterial infection in mice is provided by ATP disodium salt (50 mg/kg; ip)[4]. ?In vivo neutrophil recruitment and the release of KC, MIP-2, and IL1β are induced by ATP disodium salt[4].
|
| Animal Protocol |
Animal/Disease Models: Four weeks old Kunming mice (18-22 g)[4]
Doses: 50 mg/kg Route of Administration: intraperitoneal (ip)injection, before bacterial (E. coli) challenge Experimental Results: Protected mice from bacterial infection. |
| References |
[1]. Bonora M, et al. ATP synthesis and storage. Purinergic Signal. 2012 Sep;8(3):343-57.
[2]. M J L Bours, et al. Adenosine 5'-triphosphate and adenosine as endogenous signaling molecules in immunity and inflammation. Pharmacol Ther. 2006 Nov;112(2):358-404. [3]. Shuo Xu, et al. Doxycycline inhibits NAcht Leucine-rich repeat Protein 3 inflammasome activation and interleukin-1β production induced by Porphyromonas gingivalis-lipopolysaccharide and adenosine triphosphate in human gingival fibroblasts. Arch Oral Biol. 2019 Nov;107:104514. [4]. Yang Xiang, et al. Adenosine-5'-Triphosphate (ATP) Protects Mice against Bacterial Infection by Activation of the NLRP3 Inflammasome. PLoS One. 2013; 8(5): e63759. |
| Molecular Formula |
C10H18N5NA2O14P3
|
|---|---|
| Molecular Weight |
571.1758
|
| Exact Mass |
550.959
|
| CAS # |
987-65-5
|
| PubChem CID |
13803
|
| Appearance |
White to off-white solid powder
|
| Density |
2.63g/cm3
|
| Boiling Point |
951.4ºC at 760mmHg
|
| Melting Point |
188-190ºC
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
17
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
33
|
| Complexity |
789
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
P(=O)(O[H])(OP(=O)(O[H])OP(=O)(O[H])O[H])OC([H])([H])[C@]1([H])[C@]([H])([C@]([H])([C@]([H])(N2C([H])=NC3=C(N([H])[H])N=C([H])N=C23)O1)O[H])O[H].[Na].[Na].O([H])[H] |^1:47,48|
|
| InChi Key |
TTWYZDPBDWHJOR-IDIVVRGQSA-L
|
| InChi Code |
InChI=1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1
|
| Chemical Name |
disodium;[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-oxidophosphoryl] hydrogen phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~100 mg/mL (~181.44 mM)
DMSO : ~1 mg/mL (~1.81 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (181.44 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7508 mL | 8.7538 mL | 17.5076 mL | |
| 5 mM | 0.3502 mL | 1.7508 mL | 3.5015 mL | |
| 10 mM | 0.1751 mL | 0.8754 mL | 1.7508 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
