Size | Price | Stock | Qty |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Astragaloside I (Astrasieversianin IV; Cyclosieversioside B) is a natural product found in Astragalus membranaceus with osteogenic activity. It can stimulate osteoblast differentiation by modulating the Wnt/β-catenin signaling pathway.
ln Vitro |
In MC3T3-E1 cells, astragaloside I (10–40 μM) increases the expression of β-catenin, Runx2, BGP, and OPG, as well as RANKL (osteogenesis marker gene) [1].
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Cell Assay |
Western blot analysis[1]
Cell Types: MC3T3-E1 cells. Tested Concentrations: 0, 10, 20, 40 μM. Incubation Duration: 5 days. Experimental Results: Stimulation of β-catenin and Runx2 expression. Cytotoxicity assay [1]. Cell Types: MC3T3-E1 cells. Tested Concentrations: 0, 10, 20, 40 μM. Incubation Duration: 1, 3 or 6 days (change medium every 2 days). Experimental Results: No obvious cytotoxic effect was found on MC3T3-E1 cells. |
References |
[1]. Xun Cheng, et al. Astragaloside I Stimulates Osteoblast Differentiation Through the Wnt/β-catenin Signaling Pathway. Phytother Res. 2016 Oct;30(10):1680-1688.
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Molecular Formula |
C₄₅H₇₂O₁₆
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Molecular Weight |
869.04
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CAS # |
84680-75-1
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SMILES |
O([C@@]1([H])[C@@]([H])([C@]([H])([C@@]([H])([C@@]([H])(C([H])([H])O[H])O1)O[H])O[H])O[H])[C@@]1([H])C([H])([H])[C@@]2([H])[C@]3(C([H])([H])[H])C([H])([H])[C@@]([H])([C@]([H])([C@@]4(C([H])([H])[H])C([H])([H])C([H])([H])[C@@]([H])(C(C([H])([H])[H])(C([H])([H])[H])O[H])O4)[C@@]3(C([H])([H])[H])C([H])([H])C([H])([H])[C@]32C([H])([H])[C@]23C([H])([H])C([H])([H])[C@@]([H])(C([H])([H])C21[H])O[C@@]1([H])[C@@]([H])([C@]([H])([C@@]([H])(C([H])([H])O1)O[H])OC(C([H])([H])[H])=O)OC(C([H])([H])[H])=O)O[H]
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Synonyms |
Cyclosieversioside B Astrasieversianin IV
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~50 mg/mL (~57.53 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3.25 mg/mL (3.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3.25 mg/mL (3.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.1507 mL | 5.7535 mL | 11.5070 mL | |
5 mM | 0.2301 mL | 1.1507 mL | 2.3014 mL | |
10 mM | 0.1151 mL | 0.5753 mL | 1.1507 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.