| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
In RAW 264.7 cells, asperuloside (0-160 μg/mL, 1 hour) inhibits the production of NO, PGE2, TNF-α, and IL-6 induced by LPS [1]. In serum cell lines (U937, HL-60, AML), asperuloside (0–5 mM, 24 hours) suppresses cell viability and induces ER-dependent cellular reagents [1].
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|---|---|
| ln Vivo |
On a high-fat diet (HFD), asperuloside (orally, 3 mg/day, 0.3% of the diet, daily, for 12 weeks) decreases food intake, body weight, and fat mass [2]. In a U937 xenograft mouse model, asperuloside (30 and 60 mg/kg, intraperitoneal injection, 30 days) suppresses the formation of tumors [3]. In BALB/c mice, asperuloside (20–80 mg/kg, i.p.) inhibits MAPK and NF-κB signaling.
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| Cell Assay |
Western Blot Analysis[3]
Cell Types: U937 cells Tested Concentrations: 0-5 mM Incubation Duration: 24 h Experimental Results: Increased release of cleaved Caspase-3, Caspase-9, PARP and Cyto-c in mitochondria. Induced expression of GRP78, p-PERK, p-eIF2α, CHOP, p-IRE1, XBP1, ATF6 and cleaved Caspase-12 |
| Animal Protocol |
Animal/Disease Models: Rats eating high-fat diet (HFD) [2]
Doses: 3 mg/ Daily dosing: po, 0.3% in diet, daily, 12-week Experimental Results: weight loss, energy intake, adiposity, blood glucose, and plasma insulin. |
| References |
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| Additional Infomation |
Asperuloside is a iridoid monoterpenoid glycoside isolated from Galium verum. It has a role as a metabolite. It is an iridoid monoterpenoid, a beta-D-glucoside, a monosaccharide derivative, an acetate ester and a gamma-lactone.
Asperuloside has been reported in Oldenlandia herbacea var. herbacea, Psychotria rubra, and other organisms with data available. See also: Galium aparine whole (part of). |
| Molecular Formula |
C18H22O11
|
|---|---|
| Molecular Weight |
414.3607
|
| Exact Mass |
414.116
|
| CAS # |
14259-45-1
|
| PubChem CID |
84298
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.6±0.1 g/cm3
|
| Boiling Point |
704.2±60.0 °C at 760 mmHg
|
| Melting Point |
131-132°
|
| Flash Point |
254.1±26.4 °C
|
| Vapour Pressure |
0.0±5.1 mmHg at 25°C
|
| Index of Refraction |
1.635
|
| LogP |
-3.28
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
11
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
29
|
| Complexity |
746
|
| Defined Atom Stereocenter Count |
9
|
| SMILES |
CC(=O)OCC1=C[C@H]2[C@H]3[C@@H]1[C@@H](OC=C3C(=O)O2)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O
|
| InChi Key |
IBIPGYWNOBGEMH-DILZHRMZSA-N
|
| InChi Code |
InChI=1S/C18H22O11/c1-6(20)25-4-7-2-9-12-8(16(24)27-9)5-26-17(11(7)12)29-18-15(23)14(22)13(21)10(3-19)28-18/h2,5,9-15,17-19,21-23H,3-4H2,1H3/t9-,10+,11+,12-,13+,14-,15+,17-,18-/m0/s1
|
| Chemical Name |
[(4S,7S,8S,11S)-2-oxo-8-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,9-dioxatricyclo[5.3.1.04,11]undeca-1(10),5-dien-6-yl]methyl acetate
|
| Synonyms |
Asperuloside
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~241.34 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4134 mL | 12.0668 mL | 24.1336 mL | |
| 5 mM | 0.4827 mL | 2.4134 mL | 4.8267 mL | |
| 10 mM | 0.2413 mL | 1.2067 mL | 2.4134 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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