| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 100mg |
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| 500mg |
| ln Vitro |
Asparagusic acid is poisonous to a variety of plant parasitic nematodes and has a significant role in asparagus resistance[2]. In lettuce and other seedlings, asparagusic acid suppresses growth at 6.67×10-7 M to 6.67×10-7 M[3].
Asparagusic acid exhibited potent nematicidal activity in vitro against plant-parasitic nematodes. At a concentration of 100 μg/mL, it caused 90% mortality of the tested nematodes within 24 hours of exposure [2] Asparagusic acid (10 μM–50 μM) dose-dependently inhibited the growth of etiolated young asparagus shoots in vitro. At 50 μM, it suppressed shoot elongation by 65% and reduced fresh weight by 58% compared to the control group [3] |
|---|---|
| ln Vivo |
In etiolated young asparagus plants, Asparagusic acid (endogenous concentration ~8 μM in shoots) acted as a natural growth inhibitor, suppressing internode elongation and limiting excessive shoot growth under dark conditions [3]
In potted asparagus plants, exogenous application of Asparagusic acid (20 μM via root irrigation) reduced root colonization by plant-parasitic nematodes by 72% after 14 days, demonstrating in vivo nematicidal efficacy [2] |
| Cell Assay |
Plant cell growth inhibition assay: Etiolated asparagus shoot segments (1 cm in length) were prepared and cultured in liquid medium containing different concentrations of Asparagusic acid (10 μM–50 μM) in the dark at 25°C. After 7 days of incubation, shoot elongation length and fresh weight were measured to calculate the growth inhibition rate [3]
Nematicidal activity assay: Plant-parasitic nematodes were collected and suspended in aqueous solutions of Asparagusic acid (50 μg/mL–200 μg/mL). The nematode suspension was incubated at 25°C, and mortality was recorded at 24-hour intervals by observing nematode motility under a microscope [2] |
| References | |
| Additional Infomation |
Aspartic acid is a sulfur-containing carboxylic acid belonging to the dithiocyclopentane carboxylic acid family. It is the conjugate acid of aspartic acid. It is derived from the hydride of 1,2-dithiocyclopentane. Aspartic acid has been reported to exist in asparagus (Asparagus officinalis), and there are related data reports. Aspartic acid is a naturally occurring sulfur-containing heterocyclic compound, mainly isolated from the roots, stems and other tissues of asparagus (Asparagus officinalis)[1]. The compound has a 1,2-dithiocyclopentane ring structure, which is presumed to exert its biological activity by mediating redox reactions or interacting with the thiol groups of target biomolecules[1]. As a natural nematicide, aspartic acid plays a role in the protection of asparagus from parasitic nematode infection[2]. Asparagine acts as an endogenous plant growth regulator in asparagus, regulating stem growth under etiolation conditions to adapt to insufficient light [3].
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| Molecular Formula |
C4H6O2S2
|
|---|---|
| Molecular Weight |
150.21924
|
| Exact Mass |
149.981
|
| CAS # |
2224-02-4
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| PubChem CID |
16682
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.503g/cm3
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| Boiling Point |
323.9ºC at 760mmHg
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| Melting Point |
75.7 - 76.5 °C
|
| Flash Point |
149.7ºC
|
| Vapour Pressure |
5.19E-05mmHg at 25°C
|
| Index of Refraction |
1.645
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| LogP |
1.082
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
|
| Heavy Atom Count |
8
|
| Complexity |
98.2
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| Defined Atom Stereocenter Count |
0
|
| InChi Key |
AYGMEFRECNWRJC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C4H6O2S2/c5-4(6)3-1-7-8-2-3/h3H,1-2H2,(H,5,6)
|
| Chemical Name |
dithiolane-4-carboxylic acid
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 46 mg/mL (~306.22 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.64 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (16.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 2.5 mg/mL (16.64 mM) in 5% DMSO + 40% PEG300 + 5% Tween80 + 50% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 5: ≥ 2.5 mg/mL (16.64 mM) (saturation unknown) in 5% DMSO + 95% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 6: ≥ 0.5 mg/mL (3.33 mM) (saturation unknown) in 1% DMSO 99% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6569 mL | 33.2845 mL | 66.5690 mL | |
| 5 mM | 1.3314 mL | 6.6569 mL | 13.3138 mL | |
| 10 mM | 0.6657 mL | 3.3285 mL | 6.6569 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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