| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg | |||
| 10mg | |||
| Other Sizes |
| Targets |
Ascr#5 targets the nematode chemosensory system and the dauer formation signaling pathways. It functions as a pheromone-like cue that modulates a variety of responses in C. elegans and related species. The compound interacts with specific G protein-coupled receptors in the nematode sensory neurons, triggering downstream signaling cascades that regulate development and behavior.
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| ln Vitro |
In vitro, Ascr#5 modulates developmental and behavioral responses in C. elegans in a concentration-dependent manner. It induces dauer formation at specific concentrations and influences aggregation and mating behaviors in liquid and solid media assays. The compound is typically tested using synchronized nematode populations to assess its effects on larval development, chemotaxis, and social behaviors.
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| ln Vivo |
In vivo, Ascr#5 is a naturally occurring pheromone that regulates population density-dependent developmental decisions in C. elegans. At elevated concentrations, it promotes the formation of dauer larvae, a specialized stress-resistant stage, while at lower concentrations, it influences adult behaviors including aggregation and mating. The compound's effects are mediated through the insulin/IGF-1 and TGF-beta signaling pathways.
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| Enzyme Assay |
In vitro receptor binding assays for Ascr#5 are performed using chemosensory neurons isolated from C. elegans or using heterologous expression systems expressing candidate ascaroside receptors. Radiolabeled or fluorescently labeled Ascr#5 is incubated with neuronal preparations or receptor-expressing cells, and binding affinity is measured. Calcium imaging is used to assess receptor activation in response to Ascr#5 stimulation.
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| Cell Assay |
Cellular assays for Ascr#5 are conducted using C. elegans whole-organism platforms rather than traditional cell culture. Synchronized L1 larvae or adult worms are exposed to varying concentrations of Ascr#5 (1 nM to 100 uM) in liquid or agar plate assays. Endpoints include dauer formation rate, aggregation index, chemotaxis index, and lifespan measurement.
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| Animal Protocol |
In vivo animal experiments for Ascr#5 utilize C. elegans as the model organism. Standard protocols involve preparing agar plates containing defined concentrations of Ascr#5. Synchronized worm populations are transferred to the plates and incubated at 25degC for dauer formation assays (48-72 hours) or at 20degC for behavioral assays (1-4 hours). Dauer larvae are identified by morphology and resistance to SDS treatment.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Ascr#5 are not applicable, as the compound is used as a research tool in C. elegans and not as a therapeutic agent. The compound is stable under standard laboratory storage conditions (powder at -20degC for 3 years). For in vitro assays, Ascr#5 is typically dissolved in appropriate solvents at concentrations ranging from 1 nM to 100 uM.
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| Toxicity/Toxicokinetics |
Toxicological data for Ascr#5 are specific to its role as a nematode pheromone. At physiological concentrations, it regulates development and behavior without causing toxicity to the organism. At supra-physiological concentrations, it may induce excessive dauer formation, which is a developmental arrest state rather than a toxic effect. The compound is not known to be toxic to mammalian cells.
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| References | |
| Additional Infomation |
Ascr#5 is an ω-hydroxy fatty acid ascaroside, formed by the condensation of the hydroxyl group of 3-hydroxypropionic acid with ascarid pyranose (α-isomer). It is a major component of the diapause pheromone in Caenorhabditis elegans, acting synergistically with ascr#2 and ascr#3 as a population density signal to promote the nematode's entry into another larval stage—a highly prolonged and non-feeding diapause. It is both a pheromone and a metabolite of Caenorhabditis elegans. It is a monocarboxylic acid and an ω-hydroxy fatty acid ascaroside. Functionally, it is related to 3-hydroxypropionic acid. It is the conjugate acid of ascr#5(1-). Ascaroside C3 has been reported to exist in Caenorhabditis elegans, and relevant data are available.
Ascr#5 is a research-use compound not approved for clinical therapeutic applications. It is a critical tool in neurobiology, developmental biology, and chemical ecology research for studying C. elegans development, pheromone signaling, and population density-dependent behaviors. As a purified reference standard, it enables precise investigation of ascaroside-mediated signaling pathways. |
| Molecular Formula |
C9H16O6
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|---|---|
| Molecular Weight |
220.219743728638
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| Exact Mass |
220.095
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| CAS # |
1086696-26-5
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| PubChem CID |
44202063
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| Appearance |
White to light yellow solid powder
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| LogP |
-1.1
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
15
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| Complexity |
219
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C[C@H]1[C@@H](C[C@H]([C@@H](O1)OCCC(=O)O)O)O
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| InChi Key |
RYYMJVGKZLQYPG-YYWONIAYSA-N
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| InChi Code |
InChI=1S/C9H16O6/c1-5-6(10)4-7(11)9(15-5)14-3-2-8(12)13/h5-7,9-11H,2-4H2,1H3,(H,12,13)/t5-,6+,7+,9+/m0/s1
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| Chemical Name |
3-[(2R,3R,5R,6S)-3,5-dihydroxy-6-methyloxan-2-yl]oxypropanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~454.09 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.35 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.35 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.35 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5409 mL | 22.7046 mL | 45.4091 mL | |
| 5 mM | 0.9082 mL | 4.5409 mL | 9.0818 mL | |
| 10 mM | 0.4541 mL | 2.2705 mL | 4.5409 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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