| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
Ascr#3 targets pheromone signaling pathways in C. elegans. As an ascaroside, it functions as a chemical signal that regulates development, lifespan, and social behaviors. It acts as a male attractant and, in combination with Ascr#2, induces the formation of the dauer stage (a dormant larval stage).
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| ln Vitro |
In vitro, Ascr#3 acts as a potent male attractant in C. elegans. It promotes dauer formation when combined with Ascr#2 at low concentrations. The compound is used in behavioral assays to study nematode chemosensation and pheromone signaling. Its activity is typically assessed by measuring chemotaxis responses and dauer formation rates.
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| ln Vivo |
In vivo activity of Ascr#3 has been characterized in C. elegans. The compound induces dauer formation (dormant larval stage) and acts as a male attractant. When mixed with Ascr#2, it promotes dauer formation at low concentrations. These in vivo effects make Ascr#3 a valuable tool for studying nematode development and behavior.
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| Enzyme Assay |
In vitro receptor binding assays for Ascr#3 are not typically performed, as the compound is a pheromone that acts through chemosensory pathways in C. elegans rather than through traditional receptor binding assays. Instead, behavioral assays are used to assess its activity as a male attractant and dauer-inducing factor.
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| Cell Assay |
In vitro cellular assays for Ascr#3 are performed using C. elegans worms. Chemotaxis assays measure the attraction of male worms to the compound. Dauer formation assays assess the compound's ability to induce the dauer stage, typically in combination with Ascr#2. These assays characterize the compound's pheromone activity and its effects on nematode development.
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| Animal Protocol |
In vivo animal experiments for Ascr#3 are conducted using C. elegans. Worms are exposed to the compound, and behavioral responses such as chemotaxis are measured. Dauer formation is assessed by counting the number of worms entering the dauer stage. These experiments characterize the compound's role in nematode communication and development.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Ascr#3 have not been systematically characterized. The compound has a molecular weight of 302.36 and molecular formula C₁₅H₂₆O₆. It appears as a solid and is soluble in DMSO. The compound should be stored appropriately to maintain stability. Further PK studies are not applicable as the compound is used in C. elegans research rather than mammalian systems.
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| Toxicity/Toxicokinetics |
Toxicological data for Ascr#3 are not extensively reported. As a research-use compound used in C. elegans studies, its safety profile has not been formally evaluated in mammalian toxicology studies. The compound is intended for research purposes only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Ascr#3 is a (ω-1)-hydroxy fatty acid ascaridin, formed by the condensation of the hydroxyl group of (2E,8R)-8-hydroxynon-2-enoic acid with ascarid pyranose (α-isomer). It is a major component of the diapause pheromone in Caenorhabditis elegans, acting as a population density signal to promote its transition to another larval stage—a highly prolonged and non-feeding diapause. Furthermore, Ascr#3 synergizes with ascr#2, ascr#4, and ascr#8 to attract males. It is both a metabolite and a pheromone in Caenorhabditis elegans. It is an α,β-unsaturated monocarboxylic acid and a (ω-1)-hydroxy fatty acid ascaridin. Its function is related to (2E,8R)-8-hydroxynon-2-enoic acid. It is the conjugate acid of ascr#3(1-).
It has been reported that ascaroside C9 exists in Caenorhabditis elegans, and relevant data are available for reference. Ascr#3 is an ascaroside signaling molecule with CAS number 946524-26-1, molecular formula C₁₅H₂₆O₆, and molecular weight 302.36. It is isolated from C. elegans and acts as a potent male attractant. In combination with Ascr#2, it promotes dauer formation. It is used to study nematode communication, development, and social behaviors. |
| Molecular Formula |
C15H26O6
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|---|---|
| Molecular Weight |
302.363345623016
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| Exact Mass |
302.173
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| CAS # |
946524-26-1
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| PubChem CID |
16066476
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| Appearance |
Colorless to light yellow ointment
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| LogP |
1.449
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
21
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| Complexity |
343
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O1[C@@H](C)[C@@H](C[C@H]([C@@H]1O[C@H](C)CCCC/C=C/C(=O)O)O)O
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| InChi Key |
MWGRRKDIJLJLMO-OSYKULTDSA-N
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| InChi Code |
InChI=1S/C15H26O6/c1-10(7-5-3-4-6-8-14(18)19)20-15-13(17)9-12(16)11(2)21-15/h6,8,10-13,15-17H,3-5,7,9H2,1-2H3,(H,18,19)/b8-6+/t10-,11+,12-,13-,15-/m1/s1
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| Chemical Name |
(E,8R)-8-[(2R,3R,5R,6S)-3,5-dihydroxy-6-methyloxan-2-yl]oxynon-2-enoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~200 mg/mL (~661.46 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (16.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (16.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 5 mg/mL (16.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3073 mL | 16.5366 mL | 33.0732 mL | |
| 5 mM | 0.6615 mL | 3.3073 mL | 6.6146 mL | |
| 10 mM | 0.3307 mL | 1.6537 mL | 3.3073 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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